Western blot abreview for Anti-Calcineurin inhibitor antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole Embryo)
Gel Running Conditions
Reduced Denaturing (4%-20%)
Specification
Whole Embryo
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Room Temperature
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Santa Cruz Biotechnology
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 250 kDa
Positive control
Monoclonal Anti-Tubulin, Acetylated antibody from Sigma Aldrich @ 1:1000 dilution (Not shown in image)

Additional data

Additional Notes
Loading Amount: 3 whole mouse embryos (E9.5) were homogenized in 1mL Laemmli Buffer/Beta-Mercaptoethanol. 30uL of lysate was loaded into each well. Figure Legend: 1. New England BioLabs Prestained Protein Ladder, Broad Range 2. Cabin1 3. Cabin1 4. Cabin1 5. BioRad Precision Plus Protein Standard, Unstained

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Submitted Aug 13 2013

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