Calcineurin Phosphatase Activity Assay Kit (Colorimetric) (ab139461)

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Overview

  • Product name

    Calcineurin Phosphatase Activity Assay Kit (Colorimetric)
    See all Calcineurin kits

  • Detection method

    Colorimetric

  • Sample type

    Purified protein, Inhibitor compounds

  • Assay type

    Enzyme activity

  • Product overview

    Abcam Calcineurin Phosphatase Activity Assay Kit (Colorimetric) (ab139461) is a complete non-radioactive colorimetric assay kit designed for measuring calcineurin phosphatase activity through kinetic assay or for inhibitor screening. The activity is determined using the RII phosphopeptide substrate, the most efficient and selective peptide known for calcineurin, and detection of free phosphate released is based on the classic malachite green assay.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 x 96 tests
    96-well Clear Microplate (1/2 Volume) 1 unit
    Active Calcineurin Enzyme 1 x 50µl
    Calcineurin Assay Buffer 1 x 20ml
    Calcineurin Substrate 1 x 1.5mg
    Calmodulin 1 x 100µl
    Green Assay Reagent 1 x 20ml
    Phosphate Standard 1 x 0.5ml

  • Research areas

  • Function

    Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.

  • Sequence similarities

    Belongs to the PPP phosphatase family. PP-2B subfamily.

  • Cellular localization

    Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
  • Information by UniProt

  • Alternative names

    • Calmodulin-dependent calcineurin A subunit alpha isoform
    • CALN
    • CALNA
    • CALNA1
    • CAM-PRP catalytic subunit
    • CCN1
    • CNA1
    • PP2B
    • PP2BA_HUMAN
    • PPP2B
    • Ppp3ca
    • protein phosphatase 3, catalytic subunit, alpha isozyme
    • Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
    see all

Images

  • Standard Curve
    Standard Curve
  • Time Course
    Time Course
  • Calmodulin activation of Calcineurin Phosphatase Activity
    Calmodulin activation of Calcineurin Phosphatase Activity

    In the presence (+) of Calmodulin, Calcineurin’s activity is high. In the absence (-) of Calmodulin, Calcineurin activity of relatively low.

Protocols

References

This product has been referenced in:

  • NavaneethaKrishnan S  et al. Loss of Cdk5 in breast cancer cells promotes ROS-mediated cell death through dysregulation of the mitochondrial permeability transition pore. Oncogene 37:1788-1804 (2018). Functional Studies ; Human . Read more (PubMed: 29348461) »
  • Li Z  et al. Calcineurin/nuclear factor-?B signaling mediates isoflurane-induced hippocampal neuroinflammation and subsequent cognitive impairment in aged rats. Mol Med Rep 15:201-209 (2017). Functional Studies ; Rat . Read more (PubMed: 27909728) »
See all 3 Publications for this product

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