Product nameCalcineurin Phosphatase Activity Assay Kit (Colorimetric)
See all Calcineurin kits
Sample typePurified protein, Inhibitor compounds
Assay typeEnzyme activity
Abcam Calcineurin Phosphatase Activity Assay Kit (Colorimetric) (ab139461) is a complete non-radioactive colorimetric assay kit designed for measuring calcineurin phosphatase activity through kinetic assay or for inhibitor screening. The activity is determined using the RII phosphopeptide substrate, the most efficient and selective peptide known for calcineurin, and detection of free phosphate released is based on the classic malachite green assay.
Storage instructionsStore at -80°C. Please refer to protocols.
Components 1 x 96 tests 96-well Clear Microplate (1/2 Volume) 1 unit Active Calcineurin Enzyme 1 x 50µl Calcineurin Assay Buffer 1 x 20ml Calcineurin Substrate 1 x 1.5mg Calmodulin 1 x 100µl Green Assay Reagent 1 x 20ml Phosphate Standard 1 x 0.5ml
FunctionCalcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-2B subfamily.
Cellular localizationNucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
- Information by UniProt
- Calmodulin-dependent calcineurin A subunit alpha isoform
This product has been referenced in:
- NavaneethaKrishnan S et al. Loss of Cdk5 in breast cancer cells promotes ROS-mediated cell death through dysregulation of the mitochondrial permeability transition pore. Oncogene 37:1788-1804 (2018). Functional Studies ; Human . Read more (PubMed: 29348461) »
- Li Z et al. Calcineurin/nuclear factor-?B signaling mediates isoflurane-induced hippocampal neuroinflammation and subsequent cognitive impairment in aged rats. Mol Med Rep 15:201-209 (2017). Functional Studies ; Rat . Read more (PubMed: 27909728) »