Calcineurin Phosphatase Activity Assay Kit (Colorimetric) (ab139461)

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Standard Curve
  • Time Course
  • Calmodulin activation of Calcineurin Phosphatase Activity

Key features and details

  • Assay type: Enzyme activity
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Sample type: Inhibitor compounds, Purified protein

Overview

  • Product name

    Calcineurin Phosphatase Activity Assay Kit (Colorimetric)
    See all Calcineurin A kits

  • Detection method

    Colorimetric

  • Sample type

    Purified protein, Inhibitor compounds

  • Assay type

    Enzyme activity

  • Product overview

    Abcam Calcineurin Phosphatase Activity Assay Kit (Colorimetric) (ab139461) is a complete non-radioactive colorimetric assay kit designed for measuring calcineurin phosphatase activity through kinetic assay or for inhibitor screening. The activity is determined using the RII phosphopeptide substrate, the most efficient and selective peptide known for calcineurin, and detection of free phosphate released is based on the classic malachite green assay.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 x 96 tests
    96-well Clear Microplate (1/2 Volume) 1 unit
    Active Calcineurin Enzyme 1 x 50µl
    Calcineurin Assay Buffer 1 x 20ml
    Calcineurin Substrate 1 x 1.5mg
    Calmodulin 1 x 100µl
    Green Assay Reagent 1 x 20ml
    Phosphate Standard 1 x 0.5ml

  • Research areas

  • Function

    Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.

  • Sequence similarities

    Belongs to the PPP phosphatase family. PP-2B subfamily.

  • Cellular localization

    Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
  • Information by UniProt

  • Alternative names

    • Alpha isoform formerly PPP2B
    • Calcineurin A alpha
    • Calcineurin A1
    • CalcineurinA
    • Calmodulin dependent calcineurin A subunit alpha isoform
    • Calmodulin-dependent calcineurin A subunit alpha isoform
    • CALN
    • CALNA
    • CALNA 1
    • CALNA1
    • CAM PRP catalytic subunit
    • CAM-PRP catalytic subunit
    • CCN 1
    • CCN1
    • CNA
    • CNA 1
    • CNA alpha
    • CNA1
    • PP2BA_HUMAN
    • PPP2B
    • Ppp3ca
    • Protein phosphatase 2B catalytic subunit
    • Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform
    • Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA
    • Protein phosphatase 3 catalytic subunit alpha isozyme
    • Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform
    • Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
    see all

Images

  • Standard Curve
    Standard Curve
  • Time Course
    Time Course
  • Calmodulin activation of Calcineurin Phosphatase Activity
    Calmodulin activation of Calcineurin Phosphatase Activity

    In the presence (+) of Calmodulin, Calcineurin’s activity is high. In the absence (-) of Calmodulin, Calcineurin activity of relatively low.

References (4)

Publishing research using ab139461? Please let us know so that we can cite the reference in this datasheet.

ab139461 has been referenced in 4 publications.

  • Shiou YL  et al. Very Low-Density Lipoproteins of Metabolic Syndrome Modulates STIM1, Suppresses Store-Operated Calcium Entry, and Deranges Myofilament Proteins in Atrial Myocytes. J Clin Med 8:N/A (2019). PubMed: 31226824
  • NavaneethaKrishnan S  et al. Loss of Cdk5 in breast cancer cells promotes ROS-mediated cell death through dysregulation of the mitochondrial permeability transition pore. Oncogene 37:1788-1804 (2018). Functional Studies ; Human . PubMed: 29348461
  • Li Z  et al. Calcineurin/nuclear factor-?B signaling mediates isoflurane-induced hippocampal neuroinflammation and subsequent cognitive impairment in aged rats. Mol Med Rep 15:201-209 (2017). Functional Studies ; Rat . PubMed: 27909728
  • Li S  et al. The 1p36 Tumor Suppressor KIF 1Bß Is Required for Calcineurin Activation, Controlling Mitochondrial Fission and Apoptosis. Dev Cell 36:164-78 (2016). PubMed: 26812016

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