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    calcitonin-antibody-16b5-ab11493.pdf

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Anti-Calcitonin antibody [16B5] (ab11493)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (1)

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Sandwich ELISA - Anti-Calcitonin antibody [16B5] (ab11493)
  • Sandwich ELISA - Anti-Calcitonin antibody [16B5] (ab11493)
  • Schematic Diagram - Anti-Calcitonin antibody [16B5] (ab11493)
  • Western blot - Anti-Calcitonin antibody [16B5] (ab11493)
  • Flow Cytometry - Anti-Calcitonin antibody [16B5] (ab11493)

Key features and details

  • Mouse monoclonal [16B5] to Calcitonin
  • Suitable for: Sandwich ELISA, Flow Cyt, WB
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-Calcitonin antibody [16B5]
    See all Calcitonin primary antibodies
  • Description

    Mouse monoclonal [16B5] to Calcitonin
  • Host species

    Mouse
  • Specificity

    This antibody recognises human procalcitonin and calcitonin. Specific for calcitonin (central part).
  • Tested applications

    Suitable for: Sandwich ELISA, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Recombinant fragment (Human).

  • Epitope

    aa 72-81
  • General notes

    This product was changed from ascites to tissue culture supernatant on 13 June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from TCS.
  • Clonality

    Monoclonal
  • Clone number

    16B5
  • Myeloma

    Sp2/0
  • Isotype

    IgG2b
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Cancer
    • Growth factors
    • Hormones
    • Cancer
    • Tumor biomarkers
    • Hormones
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Recombinant Protein

    • Recombinant Human Calcitonin protein (ab153793)
  • sELISA pair antibody

    • Anti-Calcitonin antibody (ab8553)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit polyclonal to Calcitonin (ab8553).

For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab8553 as Detection.

Flow Cyt
Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

WB (1)
Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.
Notes
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit polyclonal to Calcitonin (ab8553).

For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab8553 as Detection.

Flow Cyt
Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

WB
Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.

Target

  • Relevance

    Calcitonin is a 32 amino acid peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.
  • Cellular localization

    Cytoplasmic and Secreted
  • Database links

    • Entrez Gene: 796 Human
    • Omim: 114130 Human
    • SwissProt: P01258 Human
    • Alternative names

      • Alpha CGRP antibody
      • alpha type CGRP antibody
      • CALC 1 antibody
      • CALC A antibody
      • CALC1 antibody
      • calcitonin 1 antibody
      • calcitonin gene related peptide 1 antibody
      • calcitonin gene related peptide I antibody
      • calcitonin related polypeptide alpha antibody
      • CGRP 1 antibody
      • CGRP antibody
      • CGRP I antibody
      • CGRP1 antibody
      • CT antibody
      • katacalcin antibody
      • KC antibody
      see all

    Images

    • Sandwich ELISA - Anti-Calcitonin antibody [16B5] (ab11493)
      Sandwich ELISA - Anti-Calcitonin antibody [16B5] (ab11493)

      Standard Curve for Calcitonin dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [16B5] to Calcitonin (ab11493) at 5ug/ml and Detector Antibody Rabbit polyclonal to Calcitonin (ab8553) at 0.5ug/ml

      This image was generated using the ascites version of the product.

    • Sandwich ELISA - Anti-Calcitonin antibody [16B5] (ab11493)
      Sandwich ELISA - Anti-Calcitonin antibody [16B5] (ab11493)

      Calibration curves of several procalcitonin sandwich ELISAs using a range of monclonal antibodies available agaisn't procalcitonin, calcitonin and catacalcin fragments. Capture antibodies used at 1 µg/well and detection antibodies at 0.1 µg/well.

      Dark blue square = ab11493 [16B5] (calcitonin fragment) and ab11494 [42] (procalcitonin fragment). Light grey circle = ab11497 [24B2] (calcitonin fragment) and ab11484 [13B9] (calcitonin fragment). Grey triangle = ab11487 [14C12] (catacalcin fragment) and ab11496 [14A2] (calcitonin fragment). Dark grey circle = ab14813 [27A3] (procalcitonin fragment) and ab11491 [22A11] (catacalcin fragment).

      This image was generated using the ascites version of the product.

    • Schematic Diagram - Anti-Calcitonin antibody [16B5] (ab11493)
      Schematic Diagram - Anti-Calcitonin antibody [16B5] (ab11493)

      Amino acid sequence and schematic diagram of human procalcitonin and the N-terminal, calcitonin, and katacalcin (catacalcin) fragments.

      This image was generated using the ascites version of the product.

    • Western blot - Anti-Calcitonin antibody [16B5] (ab11493)
      Western blot - Anti-Calcitonin antibody [16B5] (ab11493)

      Immunodetection of human recominbinant procalcitonin in WB by a range of monoclonal antibodies available which detect different fragments of the protein, N-terminal, calcitonin or catacalcin, (see immunogen section and related figure).

      Anti- N-terminal antibodies available; 1) ab14816 [6F10], 2) ab14813 [27A3], 3) ab11498 [38F11], 4) ab14817 [44D9], 5) ab11494 [42],

      Anti- calcitonin antibodies available; 6) ab11484 [13B9], 7) ab14818 [13F2], 8) ab14819 [13G11], 9) ab11496 [14A2], 10) ab11493 [16B5], 11) ab11497 [24B2],

      Anti- catacalcin antibodies available; 12) ab11487 [14C12], 13) ab11490 [18B7], 14) ab11491 [22A11].

      Immunodetection of human recominbinant procalcitonin in WB by a range of monoclonal antibodies available which detect different fragments of the protein, N-terminal, calcitonin or catacalcin, (see immunogen section and related figure).

      Anti- N-terminal antibodies available; 1) ab14816 [6F10], 2) ab14813 [27A3], 3) ab1149

      This image was generated using the ascites version of the product.

    • Flow Cytometry - Anti-Calcitonin antibody [16B5] (ab11493)
      Flow Cytometry - Anti-Calcitonin antibody [16B5] (ab11493)

      Overlay histogram showing SH-SY5Y cells stained with ab11493 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11493, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366<, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

      This image was generated using the ascites version of the product.

    Protocols

    • Flow cytometry protocols
    • Western blot protocols
    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab11493? Please let us know so that we can cite the reference in this datasheet.

    ab11493 has been referenced in 1 publication.

    • Sun H  et al. Expression of a chimeric human/salmon calcitonin gene integrated into the Saccharomyces cerevisiae genome using rDNA sequences as recombination sites. Appl Microbiol Biotechnol 99:10097-106 (2015). PubMed: 26254786

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews

    ELISA abreview for Anti-Calcitonin antibody [16B5 ]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Recombinant protein (Expressed in E. coli, strain BL21(DE3))
    Specification
    Expressed in E. coli, strain BL21(DE3)
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Type
    Indirect
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 13 2010

    Western blot abreview for Anti-Calcitonin antibody [16B5 ]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Escherichia coli Recombinant protein (BL21(DE3))
    Loading amount
    0.5 µg
    Specification
    BL21(DE3)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 25 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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