Product nameAnti-Calcitonin antibody
See all Calcitonin primary antibodies
DescriptionRabbit polyclonal to Calcitonin
Tested applicationsSuitable for: Sandwich ELISA, ELISA, IHC-FoFr, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Horse, Dog, Pig
Synthetic peptide within Human Calcitonin aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: P01258
- Medullary carcinoma of the thyroid or thyroid c-cell hyperplasia.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.Prolonged fixation in buffered formalin can destroy the epitope
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Light chain typeunknown
sELISA pair antibody
Our Abpromise guarantee covers the use of ab8553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [16B5] to Calcitonin (ab11493).
For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab11493 as Capture.
|ELISA||1/300 - 1/3000.|
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-P||1/300. Antigen retrieval is not essential but may optimise staining.|
|WB||1/200 - 1/500. Predicted molecular weight: 15 kDa.|
RelevanceCalcitonin is a 32 amino acid peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.
Cellular localizationCytoplasmic and Secreted
- Alpha CGRP antibody
- alpha type CGRP antibody
- CALC 1 antibody
Anti-Calcitonin antibody (ab8553) at 1/25 dilution + Human thyroid lysate.
Predicted band size: 15 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemical analysis of human thyroid staining with ab8553.
ab8553 has been referenced in 1 publication.
- Yerbury JJ et al. alpha2-Macroglobulin and haptoglobin suppress amyloid formation by interacting with prefibrillar protein species. J Biol Chem 284:4246-54 (2009). WB ; Human . PubMed: 19074141