Overview

  • Product name
    Anti-Calcitonin antibody [SP17]
    See all Calcitonin primary antibodies
  • Description
    Rabbit monoclonal [SP17] to Calcitonin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Calcitonin. The exact sequence is proprietary.
    Database link: P01258

  • Positive control
    • IHC-P: Human thyroid medullary carcinoma, pituitary and lung adenocarcinoma tissue. ICC/IF: TT cells

Properties

Applications

Our Abpromise guarantee covers the use of ab16697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
ICC/IF 1/50.

Target

  • Relevance
    Calcitonin is a 32 amino acid peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.
  • Cellular localization
    Cytoplasmic and Secreted
  • Database links
  • Alternative names
    • Alpha CGRP antibody
    • alpha type CGRP antibody
    • CALC 1 antibody
    • CALC A antibody
    • CALC1 antibody
    • calcitonin 1 antibody
    • calcitonin gene related peptide 1 antibody
    • calcitonin gene related peptide I antibody
    • calcitonin related polypeptide alpha antibody
    • CGRP 1 antibody
    • CGRP antibody
    • CGRP I antibody
    • CGRP1 antibody
    • CT antibody
    • katacalcin antibody
    • KC antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Calcitonin with purified ab16697 at 1/50 (2.4 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Formalin-fixed, paraffin-embedded human thyroid medullary carcinoma tissue stained for Calcitonin using ab16697 at 1/100 dilution in immunohistochemical analysis.

  • Ab16697 at a dilution of 1/100 staining Calcitonin in formalin fixed paraffin embedded human thyroid tissue section by Immunohistochemistry.
  • Formalin-fixed, paraffin-embedded human pituitary tissue stained for Calcitonin using ab16697 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemical analysis of paraffin-embedded Human medullary carcinoma of the thyroid gland labelling CGRP with ab137880 at 1/200 dilution.

  • Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for Calcitonin using ab16697 at 1/100 dilution in immunohistochemical analysis.

References

ab16697 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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