Overview

  • Product name
    Anti-Calcitonin receptor/CT-R antibody
    See all Calcitonin receptor/CT-R primary antibodies
  • Description
    Rabbit polyclonal to Calcitonin receptor/CT-R
  • Host species
    Rabbit
  • Specificity

    ab11042 recognises the calcitonin receptor/CT-R in human, mouse and rat. The calcitonin receptor/CT-R is a seven transmembrane domain cell surface protein which is coupled to G protein messenger systems. This antibody recognises an epitope within the cytoplasmic domain that is common to both C1a and C1b rat isoforms.

  • Tested applications
    Suitable for: IHC-Fr, IHC-FoFr, IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Rat Calcitonin receptor/CT-R aa 478-496.
    Sequence:

    GLPIYICHQEPRNPPVSNN


    Database link: P32214

  • Positive control
    • Kidney, cultered osteoclasts.
  • General notes
    Should this product contain a precipitate we recommend microcentrifugation before use.

    Previously labelled as Calcitonin receptor

Properties

Applications

Our Abpromise guarantee covers the use of ab11042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/10 - 1/50.
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/10 - 1/50. We recommend the use of 4% paraformaldehyde fixation, rather than formalin fixation. A sensitive detection system is also recommended. e.g. A catalysed signal amplification system. This product does not require protein digestion pre-treatment This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
WB Use at an assay dependent concentration. Predicted molecular weight: 56.9 kDa.
ELISA 1/10000.

Target

Images

  • ab11042 staining Calcitonin receptor/CT-R in Rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton-X, blocked with 5% serum for 45 minutes at 22°C. The sample was incubated with primary antibody (1/500) at 4°C for 18 hours. An Alexa Fluor® 488-conjugated Donkey anti-rabbit polyclonal (1/500) was used as the secondary antibody

    See Abreview

References

This product has been referenced in:
  • Goda T  et al. Calcitonin receptors are ancient modulators for rhythms of preferential temperature in insects and body temperature in mammals. Genes Dev 32:140-155 (2018). Read more (PubMed: 29440246) »
  • Langen UH  et al. Cell-matrix signals specify bone endothelial cells during developmental osteogenesis. Nat Cell Biol 19:189-201 (2017). Read more (PubMed: 28218908) »
See all 16 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain sections)
Antigen retrieval step
None
Permeabilization
Yes - 0.3% Triton-X 100
Specification
Brain sections
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Mr. Vaibhav Konanur

Verified customer

Submitted Oct 28 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up