Overview

  • Product name

    Anti-Calcitonin receptor/CT-R antibody
    See all Calcitonin receptor/CT-R primary antibodies
  • Description

    Rabbit polyclonal to Calcitonin receptor/CT-R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Calcitonin receptor/CT-R aa 445-474 (C terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    IQWNQRWGRRPSNRSARAAAAAAEAGDIPI


    Database link: P30988

  • Positive control

    • IHC-P: Human colon (vessels) tissue. ICC/IF: MCF7 cells. WB: MCF7 and A431 whole cell lysates. Flow: HL-60 cells.
  • General notes

    Previously labelled as Calcitonin receptor

Properties

Applications

Our Abpromise guarantee covers the use of ab230500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
IHC-P 1/50.
Flow Cyt 1/10 - 1/50.
ICC/IF 1/10 - 1/50.

Target

  • Function

    This is a receptor for calcitonin. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The calcitonin receptor is thought to couple to the heterotrimeric guanosine triphosphate-binding protein that is sensitive to cholera toxin.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 2 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Calcitonin receptor antibody
    • Calcitonin receptor precursor antibody
    • CALCR antibody
    • CALCR_HUMAN antibody
    • CRT antibody
    • CT R antibody
    • CT-R antibody
    • CTR antibody
    • CTR1 antibody
    see all

Images

  • All lanes : Anti-Calcitonin receptor/CT-R antibody (ab230500) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate without peptide (-)
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) cell lysate with peptide (+)
    Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate without peptide (-)
    Lane 4 : A431 (human epidermoid carcinoma cell line) cell lysate with peptide (+)

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : goat anti-rabbit(HRP) at 1/5000 dilution
  • Flow cytometric analysis of calcitonin in HL-60 (human promyelocytic leukemia cell line) cells using ab230500 at 1/10 dilution (right histogram) compared to the negetive contolr (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • MCF7 (human breast adenocarcinoma cell line) cells labelling calcitonin using ab230500 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) in ICC/IF.

    Nuclear counterstain: DAPI (blue).

  • Formalin-fixed, paraffin-embedded humsn colon (vessels) tissue stained for calcitonin using ab230500 at 1/50 dilution in immunohistochemical analysis.

References

ab230500 has not yet been referenced specifically in any publications.

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