Key features and details
- Rabbit polyclonal to Calcitonin receptor/CT-R
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Calcitonin receptor/CT-R antibody
See all Calcitonin receptor/CT-R primary antibodies
DescriptionRabbit polyclonal to Calcitonin receptor/CT-R
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human colon (vessels) tissue. ICC/IF: MCF7 cells. WB: MCF7 and A431 whole cell lysates. Flow: HL-60 cells.
Previously labelled as Calcitonin receptor
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab230500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/50.|
|ICC/IF||1/10 - 1/50.|
FunctionThis is a receptor for calcitonin. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The calcitonin receptor is thought to couple to the heterotrimeric guanosine triphosphate-binding protein that is sensitive to cholera toxin.
Sequence similaritiesBelongs to the G-protein coupled receptor 2 family.
Cellular localizationCell membrane.
- Information by UniProt
- Calcitonin receptor antibody
- Calcitonin receptor precursor antibody
- CALCR antibody
All lanes : Anti-Calcitonin receptor/CT-R antibody (ab230500) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate without peptide (-)
Lane 2 : MCF7 (human breast adenocarcinoma cell line) cell lysate with peptide (+)
Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate without peptide (-)
Lane 4 : A431 (human epidermoid carcinoma cell line) cell lysate with peptide (+)
Lysates/proteins at 35 µg per lane.
All lanes : goat anti-rabbit(HRP) at 1/5000 dilution
Flow cytometric analysis of calcitonin in HL-60 (human promyelocytic leukemia cell line) cells using ab230500 at 1/10 dilution (right histogram) compared to the negetive contolr (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
MCF7 (human breast adenocarcinoma cell line) cells labelling calcitonin using ab230500 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) in ICC/IF.
Nuclear counterstain: DAPI (blue).
Formalin-fixed, paraffin-embedded humsn colon (vessels) tissue stained for calcitonin using ab230500 at 1/50 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230500 has not yet been referenced specifically in any publications.