Overview

  • Product name

    Anti-Calcitonin receptor/CT-R antibody
    See all Calcitonin receptor/CT-R primary antibodies
  • Description

    Rabbit polyclonal to Calcitonin receptor/CT-R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Rat Calcitonin receptor/CT-R aa 450 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab93306)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Mouse small intestine; Rat kidney.
  • General notes

    Previously labelled as Calcitonin receptor

Properties

Applications

Our Abpromise guarantee covers the use of ab83297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 60 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      This is a receptor for calcitonin. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The calcitonin receptor is thought to couple to the heterotrimeric guanosine triphosphate-binding protein that is sensitive to cholera toxin.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 2 family.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Calcitonin receptor antibody
      • Calcitonin receptor precursor antibody
      • CALCR antibody
      • CALCR_HUMAN antibody
      • CRT antibody
      • CT R antibody
      • CT-R antibody
      • CTR antibody
      • CTR1 antibody
      see all

    Images

    • All lanes : Anti-Calcitonin receptor/CT-R antibody (ab83297) at 1 µg/ml

      Lane 1 : Small Intestine (Mouse) Tissue Lysate
      Lane 2 : Kidney (Rat) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 60 kDa
      Observed band size: 65 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 5 minutes


      Rat Calcitonin receptor precursor has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data), explaining the bands seen around 65 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    References

    This product has been referenced in:

    • Hughes S  et al. Expression and localisation of two-pore domain (K2P) background leak potassium ion channels in the mouse retina. Sci Rep 7:46085 (2017). IHC-Fr ; Mouse . Read more (PubMed: 28443635) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab83297.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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