Key features and details
- Assay type: Quantitative
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.1 mM
Product nameCalcium Assay Kit (Colorimetric)
See all Calcium kits
Sample typeUrine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
Sensitivity> 0.1 mM
Range0.1 mM - 25 mM
Assay time0h 20m
Species reactivityReacts with: Mammals, Other species
Calcium Assay Kit (Colorimetric) ab102505 provides a simple assay to determine calcium concentration within the physiological range of 0.4 – 100 mg/dL (0.1 – 25 mM).
In the calcium assay protocol, a chromogenic complex is formed between calcium ions and 0-cresolphthalein. The complex is measured at OD = 575 nm.
This calcium assay detects free calcium ions only.
Calcium assay protocol summary:
- add samples and standards to wells
- add chromogenic reagent, add calcium assay buffer and incubate for 5-10 min at room temp
- analyze with microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K380 Calcium Colorimetric Assay Kit. K380-250 is the same size as the 250 test size of ab102505.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 250 tests Calcium Assay Buffer NM 1 x 15ml Calcium Standard Yellow 1 x 100µl Chromogenic Reagent NM 1 x 25ml
RelevanceCalcium is essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism.
Time course of mineralization. Measurement of LDH activity. After a 15-day cultivation period of each of 2 x 106 osteoblasts growing in OSC-supplemented media (OSC+) and control osteoblasts growing in non-OSC-supplemented media (OSC-) the LDH activity in the cell free supernatants was determined as described in Material and Methods. Osteoblasts treated with 1% (v/v) Triton-X100 for 1 hour (c) served as a second control. The experiments were carried out with three primary cultures in triplicate
Effect of PFOA on calcium concentrations in cultured human osteoblasts after exposure for 3 or 5 weeks. Plots represent mean ± SD.
Ca2+ concentration (millimolar) in various biologicals. Shown bars are average of values from dilutions neat to 1:8.
Example of standard curve obtained using ab102505
ab102505 has been referenced in 59 publications.
- de Oliveira AA et al. Dissecting the interaction between HSP70 and vascular contraction: role of [Formula: see text] handling mechanisms. Sci Rep 11:1420 (2021). PubMed: 33446873
- Li Q et al. The mycoestrogen zeranol at high dosage antagonizes transient receptor potential channel activities in 3T3 L1 cells. Toxicol Lett 344:18-25 (2021). PubMed: 33689779
- Wu Y et al. Resveratrol Attenuates Oxalate-Induced Renal Oxidative Injury and Calcium Oxalate Crystal Deposition by Regulating TFEB-Induced Autophagy Pathway. Front Cell Dev Biol 9:638759 (2021). PubMed: 33718378
- Uedono H et al. Effects of fetuin-A-containing calciprotein particles on posttranslational modifications of fetuin-A in HepG2 cells. Sci Rep 11:7486 (2021). PubMed: 33820929
- Li S et al. Calcium State-Dependent Regulation of Epithelial Cell Quiescence by Stanniocalcin 1a. Front Cell Dev Biol 9:662915 (2021). PubMed: 33898465