Overview

  • Product name
    Calcium Assay Kit (Colorimetric)
    See all Calcium kits
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
  • Assay type
    Quantitative
  • Sensitivity
    > 0.1 mM
  • Range
    0.1 mM - 25 mM
  • Assay time
    0h 20m
  • Species reactivity
    Reacts with: Other species, Mammals
  • Product overview

    Calcium Assay Kit (Colorimetric) ab102505 provides a simple assay to determine calcium concentration within the physiological range of 0.4 – 100 mg/dL (0.1 – 25 mM).


    In the calcium assay protocol, a chromogenic complex is formed between calcium ions and 0-cresolphthalein. The complex is measured at OD = 575 nm.


    This calcium assay detects free calcium ions only.


    Calcium assay protocol summary:
    - add samples and standards to wells
    - add chromogenic reagent, add calcium assay buffer and incubate for 5-10 min at room temp
    - analyze with microplate reader

  • Platform
    Microplate reader

Properties

Images

  • Time course of mineralization. Measurement of LDH activity. After a 15-day cultivation period of each of 2 x 106 osteoblasts growing in OSC-supplemented media (OSC+) and control osteoblasts growing in non-OSC-supplemented media (OSC-) the LDH activity in the cell free supernatants was determined as described in Material and Methods. Osteoblasts treated with 1% (v/v) Triton-X100 for 1 hour (c) served as a second control. The experiments were carried out with three primary cultures in triplicate

  • Effect of PFOA on calcium concentrations in cultured human osteoblasts after exposure for 3 or 5 weeks. Plots represent mean ± SD.
  • Ca2+ concentration (millimolar) in various biologicals. Shown bars are average of values from dilutions neat to 1:8.

  • Example of standard curve obtained using ab102505

Protocols

References

This product has been referenced in:
  • Zhou YD & Cai L Calpeptin Reduces Neurobehavioral Deficits and Neuronal Apoptosis Following Subarachnoid Hemorrhage in Rats. J Stroke Cerebrovasc Dis 28:125-132 (2019). Read more (PubMed: 30337208) »
  • Yi J  et al. Effects of continuous and intermittent parathyroid hormone administration on midpalatal suture expansion in rats. Arch Oral Biol 99:161-168 (2019). Read more (PubMed: 30710837) »
See all 21 Publications for this product

Customer reviews and Q&As

1-10 of 25 Abreviews or Q&A

Question
Answer

Thank you for getting in touch. I am happy to provide answers to your questions:

1. According to the website the linear range of detection for this kit is between 0.1-25 mM? What about higher that?

It is possible to detect higher amount as well- however, we have not tested it and it may be above the accurate range of the assay.

2. Will other ions interfere with the measurements? Meaning presence of other divalent or monovalent ions?

ab102506 Magnesium Detection Kit did not give interference from other ions tested- Zn, Mn, Ni, Fe, Cu, Co, Ca

ab102505 Calcium Detection Kit (Colorimetric) not known to show interference from Fe+2 or Mg

3. In my solution there are also presence of MOPS ions and NaCl.  

In testing, for both kits ab102505 and ab102506 we didn’t see an issue with NaCl , however, MOPS was not tested.

4. What is the binding molecule that binds to the Ca ions? In Sigma it is the o-cresolphthalein molecule. Is the binding of your molecule overcome other interaction with other ions? Meaning that the binding is specific and the presence of other ions do not interfere with the measurement.

For the calcium assay ab102505 we have a proprietary buffer formulation that prevents interference from Fe and Mg.

5. I'm also looking to measure the concertation of Mg and Zn. I found Magnesium Assay Kit, does it works with different binding molecule? Do they interfere with one another. Is it specific as well?

6. What is the binding molecule in the Mg kit?

There are no binding molecules in the magnesium assay kit ab102506  - the assay is based on a reaction where Mg serves as a co-factor.


7. Is it possible to buy the binding molecule and not the entire kit?


Unfortunately, I am sorry this is not possible in this case.


I am happy to answer any further questions you have.

Read More

Answer


Based on the laboratories experience with the ab102505Calcium Detection Kit, for human serum samples they have either added 10 ul directly or did a 1:2 dilution with assay buffer and added 10 ul to the reaction well.

Please note that this is a guideline only and may need to be optimized by individual end users.


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Arabidopsis

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I used the calcium detection buffer to test calcium concentration in Arabidopsis, it works well.

Abcam user community

Verified customer

Submitted Jul 21 2015

Answer

I was not able to obtain the composition of the two buffers (they are considered proprietary) but the laboratory has informed me that the buffer in the calcium assay is unlikely to be compatible with the ALP activity assay.

That said, you might be able to obtain some ALP activity signal if you you use the calcium buffer for preparing your sample and then testing it for ALP activity, but at the cost of reduced sensitivity, compared to preparing the sample in the ALP kit buffer. You could test whether this will be the case by comparing standard curves using the provided ALP enzyme, prepared with each buffer.

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serum calcium in dogs

Good Good 4/5 (Ease of Use)
Abreviews
Used this product in a standard blank ELISA plate format. Programmed a DiaSorin ETI-MAX to run the procedure. Product worked as specified in dogs with no problems. Have found the curve to be very stable run-to-run.

Overall inter/intra assay precision is 5.9% and 3.6% respectively.

Randy Ringold

Verified customer

Submitted Sep 08 2014

Serum calcium in cats

Good Good 4/5 (Ease of Use)
Abreviews
Used to product to test for serum calciums in cats. Programmed a DiaSorin ETI-MAX to run the procedure on standard ELISA plates. Product performed as specified with an inter/intra assay precision of 4.9% and 3.2% respectively.

Randy Ringold

Verified customer

Submitted Sep 03 2014

Question
Answer



Ich habe nun einen Bestätigung von Labor: Der Farbstoff 0-cresolphthalein emittiert zwischen 540-600nm. Die spektralen Eigenschaften von 0-cresolphthalein hängen stark vom pH-Wert und der Bindung an Metallionen wie Calcium ab. Da für dieses Kit ein Maximum bei 575 nm erwartet wird, empfehlen wir Ihnen bei 600 nm zu messen.

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Answer



The blood vessel walls (largely Endothelial cells) samples can be homogenized in the assay buffer (contains detergent to help lyse cells) and then spun down to prepare a lysate. Several volumes of this lysate must be tested to make sure the readings are within the linear range of the std curve.

Read More

Answer

Thank you for contacting us.

The sample type of kit as mentioned on datasheet lists tissue extracts, this means the kit is suitable for any kind of tissue sections however you may need to optimize expermental conditions.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us.

This kit can be used to detect the Calcium with rat serum and cell lysates/supernatant.

To know the price please contact our Indian distributor Allied Scientific.

Allied Scientific Products
39/19B Gopal Nagar Road
Kolkata-700027
India

Tel +91 33 24566684/24793109/9039 Freephone 1800 345 7777
" tabindex="-1"> / 24790626 Emai mailto:alliedscientific@vsnl.com Websit http://www.alliedscientific.net/

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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1-10 of 25 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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