• Product name
    Calcium Assay kit (Fluorometric)
    See all Calcium kits
  • Sample type
    Serum, Plasma, Cell Lysate
  • Assay type
  • Sensitivity
    0.03 mM
  • Range
    0.04 mM - 1.5 mM
  • Assay time
    0h 30m
  • Product overview

    ab112115 provides a simple method for detecting calcium in physiology solutions by using our proprietary red fluorescence probe. The fluorescence signal can be easily read by a fluorescence microplate reader at Ex/Em = 540/590 nm. The kit can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. The assay can be completed within 30 minutes. With ab112115, we have detected as little as 0.03 mM calcium. The kit has a broad dynamic range (30 μM to 1 mM).

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    This product is intended to be used in vitro with solution-based samples such as cell extracts. If you would like to detect calcium in vivo using live cells, please check our Fluo-8, Rhod-4 or Fura-2 based assays.



  • Ca measured in biologicals showing concentration (mM)

  • Standard curve (fluorimetric): mean of duplicates (+/-SD) with background readings subtracted



This product has been referenced in:
  • White MM  et al. Neutrophil Membrane Cholesterol Content is a Key Factor in Cystic Fibrosis Lung Disease. EBioMedicine 23:173-184 (2017). Read more (PubMed: 28835336) »

See 1 Publication for this product

Customer reviews and Q&As

The assay buffer in ab112115 is 50 mM Na borate, pH = 8.5.

Component B is a simple sodium borate buffer and therefore is just a dilution buffer.

The optimum pH for this dye is pH8.5. It is quite stable as long as it is protected from the light so you should be able to observe fluorescence after 24 hou...

Read More

Thank you for contacting us.

There is always some degree of non specific binding with the fluorophore and plastic of microwell plate. We are not aware if the interaction is chemical however we recommend our customer sot use negative controls i...

Read More

Thank you for contacting us.

Unfortunately we do not have any data to prove serum interferes with the dye. However, in order to overcome this problem you could add the same amount of serum as sample into the standard.

I hope this he...

Read More

Thank you for contacting us.

In regards to your questions, we would recommend freezing the stock solution instead of the reaction mix, however, there should not be a problem if it is stored at -20C and away from light for up to a week.

Read More

Merci de nous avoir contactés.

Malheureusement, le kit ab112115 ne devrait pas être utilisé dans un milieu acide. Un tampon acide pourrait conduire à des interférences et donc diminuer la fluorescence.

Read More

Thank you for contacting us and your interest in our products.

The Calcium Quantification Assay kit (Fluorometric), ab112115, is intended for use with solution based samples. For your experiment, it may be better to have a look at the Fluo-8 M...

Read More

Thank you for contacting us.

I have brought your question to the attention of the lab who have informed me that you can wait a few hours with no effect.

I hope this information is helpful to you. Please do not hesitate to cont...

Read More

Thank you for getting back to me.

Yes, the signal would be expected to be enhanced in the presence of zinc.

And in reply to your following question, yes the Fluo-8 (ab112129) and Rhod-4 (ab112157) would not be suitable for your samp...

Read More

Thank you for contacting us yesterday in regards to the Calcium Quantification Assay kit (Fluorometric), ab112115.

I now have some further information to share with you in regards to the kit. Unfortunately, I can confirm that the kit is likel...

Read More

1-10 of 15 Abreviews or Q&A


Sign up