Key features and details
- Rabbit polyclonal to Calcium binding protein P22
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Calcium binding protein P22 antibody
See all Calcium binding protein P22 primary antibodies
DescriptionRabbit polyclonal to Calcium binding protein P22
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Orangutan
Recombinant full length protein corresponding to Human Calcium binding protein P22 aa 2-195.
GSRASTLLRDEELEEIKKETGFSHSQITRLYSRFTSLDKGENGTLSREDF QRIPELAINPLGDRIINAFFPEGEDQVNFRGFMRTLAHFRPIEDNEKSKD VNGPEPLNSRSNKLHFAFRLYDLDKDEKISRDELLQVLRMMVGVNISDEQ LGSIADRTIQEADQDGDSAISFTEFVKVLEKVDVEQKMSIRFLH
Database link: Q99653
- WB: Rat kidney and mouse brain lysates. IHC-P: Human adrenal tissue. ICC/IF: HeLa cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab223115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 22 kDa).|
FunctionRequired for constitutive membrane traffic. Inhibits GTPase-stimulated Na(+)/H(+) exchange. Also inhibits calcineurin phosphatase activity. Required for activity of SLC9A1/NHE1.
Tissue specificityUbiquitously expressed. Has been found in fetal eye, lung, liver, muscle, heart, kidney, thymus and spleen.
Sequence similaritiesContains 4 EF-hand domains.
modificationsBoth N-myristoylation and calcium-mediated conformational changes are essential for its function in exocytic traffic.
Phosphorylated; decreased phosphorylation is associated with an increase in exchange activity. The phosphorylation state may regulate the binding to NHE1.
- Information by UniProt
- Calcineurin B homolog antibody
- Calcineurin B homologous protein antibody
- Calcineurin homologous protein antibody
All lanes : Anti-Calcium binding protein P22 antibody (ab223115) at 1/500 dilution
Lane 1 : Rat kidney lysate
Lane 2 : Mouse brain lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 22 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Calcium binding protein P22 (green) using ab223115 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human adrenal gland tissue stained for Calcium binding protein P22 using ab223115 at 1/100 dilution in immunohistochemical analysis.
ab223115 has not yet been referenced specifically in any publications.