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Does your antibody work on paraffin-embedded tissue samples and is there any availability evidence of reactivity with cardiac DHPR a2 subunits? (I noticed that the Western blot trials and the background references listed on the datasheet were all done using skeletal muscle DHPR subunits).
Asked on Jan 12 2006
Thank you for your enquiry. Ab2864 detects 1,4 dihydropyridine (DHP) receptor alpha 2 subunit from human, rat, mouse, guinea pig and rabbit skeletal and cardiac muscle. To our knowledge, this antibody has yet to be tested in IHC on paraffin-embedded tissue sections. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). I hope this information helps, please do not hesitate to contact us if you need any more advice or information.
Answered on Jan 13 2006