Question (66255) | Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [20A] (ab2864)

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Question

This antibody should recognize alpha2delta1. There are 5 isoforms of this protein and the isoform 2, 4, and 5 are present in neuroblastoma cells. I have tested the presence of this protein in HEK293 cells and I found that is present. In the meantime I also tested its presence in mouse N2a and it seems to be absent. The lane is blank. I am wondering why. One possibility is that this antibody doesn't recognize the isoform present in N2a. Is that possible? What is the exact epitope of this antibody? Does it recognize all the isoforms of the protein?

Answer

Thank you for contacting us.


No epitope mapping has been completed for this product. The immunogen is rabbit dihydropyridine receptor. We do know that this product shows positive signal in Western Blot for mouse brain and IHC for rat brain. Could there be low expression in mouse N2a? Is it possible to retest with a higher ug amount on the Western Blot? I would be very happy to help work with you to achieve the best possible data using this product. If you could provide the details of your protocol including sample preparation, troubleshooting and any images, I will be very happy to review these for you.

This product is covered by our Abpromise guarantee. We are happy provide scientific support at any time. If you are using the product in species and applications listed on the datasheet and contact us within six months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue. More information on our Abpromise may be found at the following link:

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I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.



I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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