Immunohistochemistry (Frozen sections) abreview for Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [20A]

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue sections (Hindbrain section)
Permeabilization
Yes - Triton X (0.1%)
Specification
Hindbrain section
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST (0.1%) + 3% BSA
Dilution
1/1000

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa Fluor® 488 Rabbit Anti-Mouse IgG (H+L)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

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Submitted Oct 31 2013

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