Recombinant Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] - BSA and Azide free (ab273881)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23267-8] to Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 antibody [EPR23267-8] - BSA and Azide free
See all Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 primary antibodies -
Description
Rabbit monoclonal [EPR23267-8] to Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and rat skeletal muscle, brain and heart tissue lysates; C2C12, C6 and PC-12 whole cell lysates. IHC-P: Rat skeletal muscle, pancreas and cerebrum tissue; Mouse skeletal muscle tissue.
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General notes
ab273881 is the carrier-free version of ab253190.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23267-8 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab273881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 150 kDa (predicted molecular weight: 125 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 150 kDa (predicted molecular weight: 125 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Plays an important role in excitation-contraction coupling. -
Tissue specificity
Isoform 1 is expressed in skeletal muscle. Isoform 2 is expressed in the central nervous system. Isoform 2, isoform 4 and isoform 5 are expressed in neuroblastoma cells. Isoform 3, isoform 4 and isoform 5 are expressed in the aorta. -
Sequence similarities
Belongs to the calcium channel subunit alpha-2/delta family.
Contains 1 cache domain.
Contains 1 VWFA domain. -
Domain
The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch. -
Post-translational
modificationsProteolytically processed into subunits alpha-2-1 and delta-1 that are disulfide-linked. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12293 Mouse
- Entrez Gene: 25399 Rat
- SwissProt: O08532 Mouse
- SwissProt: Q14BH8 Mouse
- SwissProt: Q3TQD0 Mouse
- SwissProt: Q8C6Y3 Mouse
- SwissProt: P54290 Rat
- Unigene: 159842 Mouse
see all -
Alternative names
- CA2D1_HUMAN antibody
- CACN A2 antibody
- CACNA2 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 with ab253190 at 1/200 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on mouse skeletal muscle (PMID: 18063658). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253190).
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Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 with ab253190 at 1/200 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat cerebrum (PMID: 8955374). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253190).
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Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 with ab253190 at 1/200 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat pancreatic islet (PMID: 28115397). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253190).
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Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Calcium channel L type DHPR alpha 2 subunit/CACNA2D1 with ab253190 at 1/200 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat skeletal muscle (PMID: 18063658). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253190).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab273881 has not yet been referenced specifically in any publications.