Overview

  • Product name

    Calcium Flux Assay Kit (Flow cytometry)
    See all Calcium kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based (quantitative)
  • Product overview

    Calcium Flux Assay Kit (Flow cytometry) (ab233472) is a fluorescence-based assay for detecting intracellular calcium mobilization using a flow cytometer. It can be used for kinetic reading or for endpoint reading


    After loading the 520 AM dye into the cells of interest, simply wash the cells and add the calcium flux agonist then read the sample via a flow cytometer using kinetic reading mode or endpoint reading mode at Ex/Em = 490/525 nm.

  • Platform

    Flow cytometer

Properties

Images

  • The ATP dose dependent (10 µM, 1 µM or 0 µM ATP) intracellular calcium release was measured by Calcuim Flux Assay Kit (Flow cytometry) (ab233472) in CHO-K1 cells.

    Cells were incubated with 520 AM dye for 30 minutes at 37 °C before ATP was added into the cells. The baseline was acquired and the rest of the cells were analyzed after the addition of ATP. The response was measured over time. The analysis was done on NovoCyte™ 3000 Flow Cytometer.

Protocols

References

ab233472 has not yet been referenced specifically in any publications.

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