Product nameAnti-Calcium-independent Phospholipase A2/PLA2G6 antibody
See all Calcium-independent Phospholipase A2/PLA2G6 primary antibodies
DescriptionRabbit polyclonal to Calcium-independent Phospholipase A2/PLA2G6
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment corresponding to Human Calcium-independent Phospholipase A2/PLA2G6 aa 100-250.
QVLHTEVLQHLTDLIRNHPSWSVAHLAVELGIRECFHHSRIISCANCAEN EEGCTPLHLACRKGDGEILVELVQYCHTQMDVTDYKGETVFHYAVQGDNS QVLQLLGRNAVAGLNQVNNQGLTPLHLACQLGKQEMVRVLLLCNARCNIM G
Database link: O60733
- WB: HEL cell lysate. Calcium-independent Phospholipase A2/PLA2G6 overexpression HEK-293T cell lysate. ICC/IF: A431 cells.
This product was previously labelled as Calcium-independent Phospholipase A2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.4 µg/ml. Predicted molecular weight: 90 kDa.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionCatalyzes the release of fatty acids from phospholipids. It has been implicated in normal phospholipid remodeling, nitric oxide-induced or vasopressin-induced arachidonic acid release and in leukotriene and prostaglandin production. May participate in fas mediated apoptosis and in regulating transmembrane ion flux in glucose-stimulated B-cells. Has a role in cardiolipin (CL) deacylation. Required for both speed and directionality of monocyte MCP1/CCL2-induced chemotaxis through regulation of F-actin polymerization at the pseudopods.
Isoform ankyrin-iPLA2-1 and isoform ankyrin-iPLA2-2, which lack the catalytic domain, are probably involved in the negative regulation of iPLA2 activity.
Tissue specificityFour different transcripts were found to be expressed in a distinct tissue distribution.
Involvement in diseaseNeurodegeneration with brain iron accumulation 2B
Neurodegeneration with brain iron accumulation 2A
Parkinson disease 14
Sequence similaritiesContains 7 ANK repeats.
Contains 1 patatin domain.
Cellular localizationCytoplasm and Membrane. Recruited to the membrane-enriched pseudopod upon MCP1/CCL2 stimulation in monocytes.
- Information by UniProt
FormThe isoform LH-iPLA2 is located at the membrane. The isoform SH-iPLA2 is cytoplasmic.
- 85 kDa calcium independent phospholipase A2 antibody
- 85/88 kDa calcium-independent phospholipase A2 antibody
- BB112799 antibody
PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling Calcium-independent Phospholipase A2/PLA2G6 using ab244247 at 4 µg/ml (green) in ICC/IF.
Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody (ab244247) at 0.4 µg/ml + HEL (Human bone marrow erythroleukemia cell line) cell lysate
Predicted band size: 90 kDa
All lanes : Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody (ab244247) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Calcium-independent Phospholipase A2 overexpression HEK-293T cell lysate
Predicted band size: 90 kDa
ab244247 has not yet been referenced specifically in any publications.