Anti-Calcium Pump PMCA2 ATPase antibody (ab3529)
Key features and details
- Rabbit polyclonal to Calcium Pump PMCA2 ATPase
- Suitable for: IHC-FoFr, ICC, ELISA, IHC-Fr, WB, ICC/IF, IHC-P, IP
- Reacts with: Mouse, Rat, Chicken, Human, Pig, Non human primates
- Isotype: IgG
Overview
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Product name
Anti-Calcium Pump PMCA2 ATPase antibody -
Description
Rabbit polyclonal to Calcium Pump PMCA2 ATPase -
Host species
Rabbit -
Specificity
Detects PMCA 2a and 2b ATPase. -
Tested applications
Suitable for: IHC-FoFr, ICC, ELISA, IHC-Fr, WB, ICC/IF, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Human, Pig, Non human primates -
Immunogen
Synthetic peptide corresponding to Human Calcium Pump PMCA2 ATPase aa 1-100.
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Positive control
- IHC-P: Human brain and cervical carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-FoFr | (1) |
Use at an assay dependent concentration.
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ICC |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 137 kDa.
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ICC/IF |
Use at an assay dependent concentration. PubMed: 20489728
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IHC-P | (1) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Notes |
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IHC-FoFr
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 137 kDa. |
ICC/IF
Use at an assay dependent concentration. PubMed: 20489728 |
IHC-P
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Target
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Function
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. -
Tissue specificity
Mainly expressed in brain cortex. Found in low levels in skeletal muscle, heart muscle, stomach, liver, kidney and lung. Isoforms containing segment B are found in brain cortex and at low levels in other tissues. Isoforms containing segments X and W are found at low levels in all tissues. Isoforms containing segment A and segment Z are found at low levels in skeletal muscle and heart muscle. -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 491 Human
- Entrez Gene: 11941 Mouse
- Entrez Gene: 24215 Rat
- Omim: 108733 Human
- SwissProt: Q01814 Human
- SwissProt: Q9R0K7 Mouse
- SwissProt: P11506 Rat
- Unigene: 268942 Human
see all -
Alternative names
- AT2B2_HUMAN antibody
- atp2b2 antibody
- ATPase plasma membrane Ca2+ transporting 2 antibody
see all
Images
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Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing PMCA2 ATPase ab3529 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Human cerebellum (Centre panel) and lung (Negative control, right panel) stained for PMCA2 ATPase using ab3529 at 1/20 dilution in immunohistochemical analysis. The left hand panel is a secondary antibody only control.
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Immunohistochemistry was performed on cancer biopsies of deparaffinized Human cervical carcinoma tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA2 ATPase ab3529 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Protocols
Datasheets and documents
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Datasheet download
References (13)
ab3529 has been referenced in 13 publications.
- Ikäheimo K et al. MANF supports the inner hair cell synapse and the outer hair cell stereocilia bundle in the cochlea. Life Sci Alliance 5:N/A (2022). PubMed: 34815294
- Newton S et al. Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing. PLoS Genet 18:e1009937 (2022). PubMed: 35100259
- Lewis MA et al. Identification and characterisation of spontaneous mutations causing deafness from a targeted knockout programme. BMC Biol 20:67 (2022). PubMed: 35296311
- Ilic K et al. Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts. Int J Mol Sci 22:N/A (2021). PubMed: 34948386
- Robertson SYT et al. Multiple Calcium Export Exchangers and Pumps Are a Prominent Feature of Enamel Organ Cells. Front Physiol 8:336 (2017). WB . PubMed: 28588505