Overview

  • Product name

    Anti-Calcium Pump PMCA2 ATPase antibody
  • Description

    Rabbit polyclonal to Calcium Pump PMCA2 ATPase
  • Host species

    Rabbit
  • Specificity

    Detects PMCA 2a and 2b ATPase.
  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, IP, WB, ELISA, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Human, Pig, Non human primates
  • Immunogen

    Synthetic peptide corresponding to Human Calcium Pump PMCA2 ATPase aa 5-19.
    Sequence:

    TNSDFYSKNQRNESS

  • Positive control

    • WB: Human and rat brain tissue lysates. IHC-P: Human brain and cervical carcinoma tissues. Mouse purkinje cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20489728
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/20 - 1/200.
ICC Use at an assay dependent concentration.
IP Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 137 kDa (predicted molecular weight: 137 kDa).
ELISA Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function

    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.
  • Tissue specificity

    Mainly expressed in brain cortex. Found in low levels in skeletal muscle, heart muscle, stomach, liver, kidney and lung. Isoforms containing segment B are found in brain cortex and at low levels in other tissues. Isoforms containing segments X and W are found at low levels in all tissues. Isoforms containing segment A and segment Z are found at low levels in skeletal muscle and heart muscle.
  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AT2B2_HUMAN antibody
    • atp2b2 antibody
    • ATPase plasma membrane Ca2+ transporting 2 antibody
    • Plasma membrane calcium ATPase 2 antibody
    • Plasma membrane calcium ATPase antibody
    • Plasma membrane calcium ATPase isoform 2 antibody
    • Plasma membrane calcium pump isoform 2 antibody
    • Plasma membrane calcium transporting ATPase 2 antibody
    • Plasma membrane calcium-transporting ATPase 2 antibody
    • PMCA2 antibody
    • PMCA2a antibody
    • PMCA2i antibody
    see all

Images

  • All lanes : Anti-Calcium Pump PMCA2 ATPase antibody (ab3529) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 137 kDa


    Exposure time: 2 minutes


    There are 8 isoforms of Calcium Pump PMCA2 ATPase (Swissprot). Ab3529 is expected to recognise all isoforms. The predicted molecular weight of the different isoforms ranges from 127-kDa to 138-kDa. The detection of more than one isoform by ab3529 in brain tissue lysates may explain the banding pattern observed. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • ab3529 staining Calcium Pump PMCA2 ATPase in Mouse purkinje cells in cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/500 in PBS + 3% NGS + 0.5% Triton X-100) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG monoclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing PMCA2 ATPase ab3529 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on cancer biopsies of deparaffinized Human cervical carcinoma tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA2 ATPase ab3529 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

References

This product has been referenced in:

  • Robertson SYT  et al. Multiple Calcium Export Exchangers and Pumps Are a Prominent Feature of Enamel Organ Cells. Front Physiol 8:336 (2017). WB . Read more (PubMed: 28588505) »
  • Schmidt N  et al. Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca2+-ATPases and Key Regulators of Ca2+ Clearance. Neuron 96:827-838.e9 (2017). Read more (PubMed: 29056295) »
See all 9 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
None
Sample
Mouse Tissue sections (Purkinje cells in cerebellum)
Specification
Purkinje cells in cerebellum
Permeabilization
Yes - 0.5% Triton x100
Fixative
Paraformaldehyde

Dr. Pradeep Sherkhane

Verified customer

Submitted Jul 08 2013

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue lysate - whole (Rat cerebellar slices)
Specification
Rat cerebellar slices
Fixative
Formaldehyde
Antigen retrieval step
None
Blocking step
Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1%

Abcam user community

Verified customer

Submitted Aug 17 2005

Application
Western blot
Sample
Rat Tissue lysate - other (Rat forebrain and cerebellum preparations, eg syna)
Specification
Rat forebrain and cerebellum preparations, eg syna
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Aug 17 2005

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