Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue lysate - whole (Rat cerebellar slices)
Specification
Rat cerebellar slices
Fixative
Formaldehyde
Antigen retrieval step
None
Blocking step
Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1%
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Molecular probes goat anti-rabbit Alexa Fluor488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Blocking was performed with premeabilisation using 1% goat serum, 0.5% BSA and 0.5% Triton X-100 for 4 hours at 37 degrees. Primary antibody incubation was performed overnight at room temperature ina humidified chamber.
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Submitted Aug 17 2005