Anti-Calcium Pump PMCA3 ATPase antibody (ab154213)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Calcium Pump PMCA3 ATPase antibody
See all Calcium Pump PMCA3 ATPase primary antibodies -
Description
Rabbit polyclonal to Calcium Pump PMCA3 ATPase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment, corresponding to a region within internal sequence amino acids 560-887 of Human Calcium Pump PMCA3 ATPase (Uniprot ID: Q16720).
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Positive control
- U-87 MG whole cell lysate; Human U87 xenograft tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab154213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 134 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Relevance
Calcium Pump PMCA3 ATPase belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. Calcium Pump PMCA3 ATPase is the plasma membrane calcium ATPase isoform 3. -
Cellular localization
Cell membrane; Multi-pass membrane protein. -
Database links
- Entrez Gene: 504353 Cow
- Entrez Gene: 492 Human
- Entrez Gene: 320707 Mouse
- Entrez Gene: 29599 Rat
- Omim: 300014 Human
- SwissProt: Q16720 Human
- SwissProt: A2ALL9 Mouse
- SwissProt: Q0VF54 Mouse
see all -
Alternative names
- ATP2B3 antibody
- ATP2B3 protein antibody
- ATPase Ca++ transporting plasma membrane 3 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (ab154213)Immunohistochemical analysis of paraffin-embedded Human U87 xenograft tissue, labeling Calcium Pump PMCA3 ATPase with ab154213 at 1/500 dilution. -
Western blot - Anti-Calcium Pump PMCA3 ATPase antibody (ab154213)Anti-Calcium Pump PMCA3 ATPase antibody (ab154213) at 1/1000 dilution + U-87 MG whole cell lysate at 30 µg
Predicted band size: 134 kDa
5% SDS PAGE
References
ab154213 has not yet been referenced specifically in any publications.
