Key features and details
- Rabbit polyclonal to Calcium Pump PMCA3 ATPase
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Calcium Pump PMCA3 ATPase antibody
See all Calcium Pump PMCA3 ATPase primary antibodies
DescriptionRabbit polyclonal to Calcium Pump PMCA3 ATPase
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- IHC-P: Human cerebellum tissue. ICC/IF: A431 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
RelevanceCalcium Pump PMCA3 ATPase belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. Calcium Pump PMCA3 ATPase is the plasma membrane calcium ATPase isoform 3.
Cellular localizationCell membrane; Multi-pass membrane protein.
- ATP2B3 antibody
- ATP2B3 protein antibody
- ATPase Ca++ transporting plasma membrane 3 antibody
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for Calcium Pump PMCA3 ATPase (green) using ab223787 at 4 µg/ml in ICC/IF.
Paraffin-embedded human cerebellum tissue stained for Calcium Pump PMCA3 ATPase using ab223787 at 1/200 dilution in immunohistochemical analysis.
ab223787 has not yet been referenced specifically in any publications.