Anti-Calcium Pump PMCA3 ATPase antibody (ab3530)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-Calcium Pump PMCA3 ATPase antibody
See all Calcium Pump PMCA3 ATPase primary antibodies -
DescriptionRabbit polyclonal to Calcium Pump PMCA3 ATPase
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Host speciesRabbit
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SpecificityDetects PMCA 3a and 3b ATPase.
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Tested applicationsSuitable for: IHC-Fr, IP, WB, ELISA, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human, Pig, Non human primates
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Immunogen
Synthetic peptide corresponding to Rat Calcium Pump PMCA3 ATPase aa 5-22.
Sequence:ANSSIEFHPKPQQQREVP
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Positive control
- WB: rat brain microsomal fractions.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab3530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | 1/20. | |
| IP | Use a concentration of 1 µg/ml. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 127 kDa (predicted molecular weight: 134 kDa). This antibody detects an ~127 kDa protein representing PMCA 3a and PMCA 3b ATPase from rat brain microsomal fractions. |
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| ELISA | Use at an assay dependent concentration. | |
| IHC-P | 1/20. |
Target
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RelevanceCalcium Pump PMCA3 ATPase belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. Calcium Pump PMCA3 ATPase is the plasma membrane calcium ATPase isoform 3.
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Cellular localizationCell membrane; Multi-pass membrane protein.
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Database links
- Entrez Gene: 492 Human
- Entrez Gene: 320707 Mouse
- Entrez Gene: 29599 Rat
- Omim: 300014 Human
- SwissProt: Q16720 Human
- SwissProt: A2ALL9 Mouse
- SwissProt: Q0VF54 Mouse
- SwissProt: Q0VF55 Mouse
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Alternative names
- ATP2B3 antibody
- ATP2B3 protein antibody
- ATPase Ca++ transporting plasma membrane 3 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (ab3530)This image is courtesy of an Abreview submitted by Dr R Empsonab3530 at 1/200 staining rat hippocampal slices and slice cultures by IHC-P. The tissue was paraformaldehyde fixed, permeabilized with Triton X-100 and blocked with BSA before incubating with the antibody for 12 hours. An Alexa Fluor ®488 conjugated goat anti rabbit antibody was used secondary.
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Western blot - Anti-Calcium Pump PMCA3 ATPase antibody (ab3530)Image courtesy of an anonymous Abreview.All lanes : Anti-Calcium Pump PMCA3 ATPase antibody (ab3530) at 1/1000 dilution
All lanes : Whole cell lysate prepared from rat primary cortical neurons
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated secondary at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 134 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (ab3530)Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA3 ATPase ab3530 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (ab3530)Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA3 ATPase ab3530 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Datasheets and documents
References
This product has been referenced in:
- Robertson SYT et al. Multiple Calcium Export Exchangers and Pumps Are a Prominent Feature of Enamel Organ Cells. Front Physiol 8:336 (2017). WB . Read more (PubMed: 28588505) »
- Figueroa KP et al. Spontaneous shaker rat mutant - a new model for X-linked tremor/ataxia. Dis Model Mech 9:553-62 (2016). WB, IHC . Read more (PubMed: 27013529) »
