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Neuroscience Neurotransmission Calcium Signaling Calcium Pump
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Anti-Calcium Pump PMCA3 ATPase antibody (ab3530)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (5)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (ab3530)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (ab3530)

Key features and details

  • Rabbit polyclonal to Calcium Pump PMCA3 ATPase
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Calcium Pump PMCA3 ATPase antibody
    See all Calcium Pump PMCA3 ATPase primary antibodies
  • Description

    Rabbit polyclonal to Calcium Pump PMCA3 ATPase
  • Host species

    Rabbit
  • Specificity

    Detects PMCA 3a and 3b ATPase.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Rat Calcium Pump PMCA3 ATPase aa 1-100.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calcium Pump
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Channels
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
Application Abreviews Notes
IHC-P (1)
1/20.
Notes
IHC-P
1/20.

Target

  • Relevance

    Calcium Pump PMCA3 ATPase belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. Calcium Pump PMCA3 ATPase is the plasma membrane calcium ATPase isoform 3.
  • Cellular localization

    Cell membrane; Multi-pass membrane protein.
  • Database links

    • Entrez Gene: 492 Human
    • Omim: 300014 Human
    • SwissProt: Q16720 Human
    • Alternative names

      • ATP2B3 antibody
      • ATP2B3 protein antibody
      • ATPase Ca++ transporting plasma membrane 3 antibody
      • ATPase plasma membrane Ca2+ transporting 3 antibody
      • Plasma membrane calcium ATPase 3 antibody
      • Plasma membrane calcium ATPase isoform 3 antibody
      • Plasma membrane calcium pump isoform 3 antibody
      • Plasma membrane calcium transporting ATPase 3 antibody
      • PMCA 3 antibody
      • PMCA3 antibody
      • PMCA3a antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (ab3530)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (ab3530)
      Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA3 ATPase ab3530 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (ab3530)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (ab3530)
      Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA3 ATPase ab3530 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (5)

    Publishing research using ab3530? Please let us know so that we can cite the reference in this datasheet.

    ab3530 has been referenced in 5 publications.

    • Zhang J  et al. Priority Strategy of Intracellular Ca2+ Homeostasis in Skeletal Muscle Fibers During the Multiple Stresses of Hibernation. Cells 9:N/A (2019). PubMed: 31877883
    • Robertson SYT  et al. Multiple Calcium Export Exchangers and Pumps Are a Prominent Feature of Enamel Organ Cells. Front Physiol 8:336 (2017). WB . PubMed: 28588505
    • Figueroa KP  et al. Spontaneous shaker rat mutant - a new model for X-linked tremor/ataxia. Dis Model Mech 9:553-62 (2016). WB, IHC . PubMed: 27013529
    • Kosiorek M  et al. Interaction of plasma membrane Ca(2+)-ATPase isoform 4 with calcineurin A: Implications for catecholamine secretion by PC12 cells. Biochem Biophys Res Commun 411:235-40 (2011). WB ; Rat . PubMed: 21740891
    • Jensen TP  et al. Presynaptic plasma membrane Ca2+ ATPase isoform 2a regulates excitatory synaptic transmission in rat hippocampal CA3. J Physiol 579:85-99 (2007). WB ; Rat . PubMed: 17170045

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-Calcium Pump PMCA3 ATPase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (primary cortical neurons)
    Loading amount
    20 µg
    Specification
    primary cortical neurons
    Gel Running Conditions
    Reduced Denaturing
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 22 2008

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calcium Pump PMCA3 ATPase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Rat hippocampal slices and slice cultures)
    Specification
    Rat hippocampal slices and slice cultures
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    Yes - 1% Triton X-100
    Blocking step
    BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
    Read More

    Dr. R Empson

    Verified customer

    Submitted Aug 09 2007

    Western blot abreview for Anti-Calcium Pump PMCA3 ATPase antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Rat forebrain or cerebellar homogenates)
    Specification
    Rat forebrain or cerebellar homogenates
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 17 2005

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