Overview

  • Product name

    Anti-Calcium Pump PMCA4 ATPase antibody
    See all Calcium Pump PMCA4 ATPase primary antibodies
  • Description

    Rabbit polyclonal to Calcium Pump PMCA4 ATPase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Calcium Pump PMCA4 ATPase aa 281-345.
    Database link: P23634

  • Positive control

    • IHC-P: Human colon cancer and brain tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab238158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/20 - 1/200.
IHC-P 1/20 - 1/200.

Target

  • Function

    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.
  • Tissue specificity

    Isoform XB is the most abundant isoform and is expressed ubiquitously. Isoforms containing segment Z have only been detected in heart, while isoforms containing segment a have been found in heart, stomach and brain cortex.
  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AT2B4_HUMAN antibody
    • ATP2B2 antibody
    • Atp2b4 antibody
    • ATPase Ca++ transporting plasma membrane 4 antibody
    • Cation transporting ATPase antibody
    • DKFZp686G08106 antibody
    • DKFZp686M088 antibody
    • Matrix remodelling associated 1 antibody
    • Matrix remodelling associated protein 1 antibody
    • Matrix-remodeling-associated protein 1 antibody
    • MXRA 1 antibody
    • MXRA1 antibody
    • OTTHUMP00000034088 antibody
    • OTTHUMP00000034089 antibody
    • Plasma membrane calcium ATPase 4 antibody
    • Plasma membrane calcium ATPase isoform 4 antibody
    • Plasma membrane calcium pump isoform 4 antibody
    • Plasma membrane calcium transporting ATPase 4 antibody
    • Plasma membrane calcium-transporting ATPase 4 antibody
    • PMCA 4 antibody
    • PMCA4 antibody
    • PMCA4b antibody
    • PMCA4x antibody
    • Sarcolemmal calcium pump antibody
    see all

Images

  • HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Calcium Pump PMCA4 ATPase (Green) using ab238158 at a 1/33 dilution in ICC/IF.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

  • Paraffin-embedded human colon cancer tissue stained for Calcium Pump PMCA4 ATPase with ab238158 at a 1/100 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Paraffin-embedded human brain tissue stained for Calcium Pump PMCA4 ATPase with ab238158 at a 1/100 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

References

ab238158 has not yet been referenced specifically in any publications.

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