Overview

  • Product name
    Anti-Calcium Sensing Receptor antibody [5C10, ADD]
    See all Calcium Sensing Receptor primary antibodies
  • Description
    Mouse monoclonal [5C10, ADD] to Calcium Sensing Receptor
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, WB, IHC-FoFr, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Pig
  • Immunogen

    Synthetic peptide:

    ADDDYGRPGIEKFREEAEERDI

    , corresponding to amino acids 214-235 of Human Calcium Sensing Receptor.

  • Positive control
    • Detects human, bovine and rat calcium sensing receptor.

Properties

Applications

Our Abpromise guarantee covers the use of ab19347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

WB Use a concentration of 2 µg/ml. Detects a band of approximately 130, 150 kDa.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19056349
ELISA Use at an assay dependent concentration.
IHC-Fr Use a concentration of 2 µg/ml.

Target

  • Function
    Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system.
  • Tissue specificity
    Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta.
  • Involvement in disease
    Defects in CASR are the cause of familial hypocalciuric hypercalcemia type 1 (FHH) [MIM:145980]. FHH is characterized by altered calcium homeostasis. Affected individuals exhibit mild or modest hypercalcemia, relative hypocalciuria, and inappropriately normal PTH levels.
    Defects in CASR are the cause of neonatal severe primary hyperparathyroidism (NSHPT) [MIM:239200]. NSHPT is a rare autosomal recessive life-threatening disorder characterized by very high serum calcium concentrations, skeletal demineralization, and parathyroid hyperplasia. In some instances NSHPT has been demonstrated to be the homozygous form of FHH.
    Defects in CASR are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. An autosomal recessive form of FIH also exists.
    Defects in CASR are the cause of idiopathic generalized epilepsy type 8 (IGE8) [MIM:612899]; also known as EIG8. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Seizure types are variable, but include myoclonic seizures, absence seizures, febrile seizures, complex partial seizures, and generalized tonic-clonic seizures.
    Note=Homozygous defects in CASR can be a cause of primary hyperparathyroidism in adulthood. Patients suffer from osteoporosis and renal calculi, have marked hypercalcemia and increased serum PTH concentrations.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 3 family.
  • Post-translational
    modifications
    N-glycosylated.
    Ubiquitinated by RNF19A; which induces proteasomal degradation.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ca sensing receptor antibody
    • Ca2+ sensing receptor 1 antibody
    • Ca2+ sensing receptor antibody
    • Calcium sensing receptor antibody
    • CAR antibody
    • CaSR antibody
    • CASR_HUMAN antibody
    • EIG8 antibody
    • Extracellular calcium sensing receptor antibody
    • Extracellular calcium sensing receptor [Precursor] antibody
    • Extracellular calcium-sensing receptor antibody
    • FHH antibody
    • FIH antibody
    • GPRC2A antibody
    • HHC antibody
    • HHC1 antibody
    • Hypocalciuric hypercalcemia 1 antibody
    • Hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism antibody
    • MGC138441 antibody
    • NSHPT antibody
    • Parathyroid Ca(2+) sensing receptor 1 antibody
    • Parathyroid Cell calcium sensing receptor antibody
    • Parathyroid Cell calcium-sensing receptor antibody
    • PCAR 1 antibody
    • PCaR1 antibody
    see all

Images

  • Immunohistochemical analysis of 4% formalin fixed frozen mouse stomach tissue labeling Calcium Sensing Receptor with ab19347 at 1/100 dilution overnight at 4°C, followed by fluorophore-conjugated goat anti-mouse IgG secondary antibody for 2 hours at RT.

    Fresh stomach tissue was fixed in 4% formalin for 1 hour and then incubated overnight at  in 25% sucrose before embedding in tissue freezing medium. Antigen retrieval was carried out on 8µm cryo-sections by incubating in sodium citrate buffer for 45 minutes at 4°C, immersing in sodium citrate buffer for 10 minutes at 100°C before washing 3 times for 5 minutes each in 1X PBS. Sections were then blocked in blocking buffer (0.3% Triton X-100 in 1X PBS containing 10% normal goat serum) for 30 minutes at RT before staining with ab19347.

    Sections were also stained with DAPI nuclear stain (blue). Positive cells (green) were found at the base of the antral glands in the mouse stomach.

     

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Calcium Sensing Receptor ab19347 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Overlay histogram showing SH-SY5Y cells stained with ab19347 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19347, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed SH-SY5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Calcium Sensing Receptor ab19347 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • ab19347 at 1/100 staining rat brain (cerebral cortex) tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum before incubation with the primary antibody for 24 hours at 4°C. A biotinylated horse anti-mouse IgG was used as the secondary.

    See Abreview

References

This product has been referenced in:
  • Jiang P  et al. The Expression of Calcium Sensing Receptor in Normal and Diabetic Rat Eyes. Med Sci Monit 24:706-710 (2018). Read more (PubMed: 29397395) »
  • Gorvin CM  et al. Cinacalcet Rectifies Hypercalcemia in a Patient With Familial Hypocalciuric Hypercalcemia Type 2 (FHH2) Caused by a Germline Loss-of-Function Ga11 Mutation. J Bone Miner Res 33:32-41 (2018). WB . Read more (PubMed: 28833550) »
See all 29 Publications for this product

Customer reviews and Q&As

1-10 of 15 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Joint knee)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Specification
Joint knee
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 24°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 28 2018

Application
Immunohistochemistry (Frozen sections)
Blocking step
1% BSA + 5% NGS as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Sample
Human Tissue sections (blood vessels)
Specification
blood vessels
Permeabilization
No
Fixative
Paraformaldehyde

Mr. Florian Guilbaud

Verified customer

Submitted Mar 24 2014

Question
Answer

Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
I look forward to receiving your reply.

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Question
Answer

Thank you for your emails and telephone calls today.

I am very pleased to hear you would like to accept our offer and test ab19347 in IHC-P. I have arranged the following discount code:

DISCOUNT CODE: #####
Expiration date: #####


This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab19347 in IHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question
Answer

Thank you for your telephone enquiry earlier this morning.

As discussed on the telephone, I am sorry to conrirm that to our knowledge, ab19347 has not been tested in WB. Therefore, I can offer a discount off a future purchase if you buy ab19347 now, test it in WB and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab19347 in WB. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering.

2. Purchase ab19347 either by phone, fax, or online (www.abcam.com).

3. Test it in WB.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab19347 turns out to be unsuitable for WB, you will still receive the discount on your next purchase after your Abreview has been submitted.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount

Please let me know if you have any questions about this offer and I would be happy to help you further.

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Answer

DISCOUNT CODE: xxx for ab19347
DISCOUNT CODE: xxx for ab71842
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab19347 and ab71842in chicken and/or IHC-Fr. Each code will give you: 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for chicken and/or IHC-Frand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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Question
Answer

Thank you for contacting us.

The catalogue number of blocking peptide is ab18608.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for your enquiry and your interest in our products.

I can confirm it for you that this antibody (ab19347: Anti-Calcium Sensing Receptor antibody) has been tested for cross-reaction with rat, cow, human. Based on blast search (using Uniprot) there is a very high homology between human and bovine aa for Calcium Sensing Receptor. Unfortunately, there seems to be no information on procine CaSR on the public domain, you may wish to check this website for further information:

http://www.uniprot.org/blast/uniprot/2012032742K5NXXKK7

If you wish to test this antibody in pig samples, I could offer you a testing discount. The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

I hope this helps and if I can assist further, please do not hesitate to contact me.

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Answer

Thank you for contacting us again. I am very pleased to hear you are choosing to test ab18200 with your mouse samples. I have generated the discount code, this will entitle you to one free primary antibody before the expiration date: DISCOUNT CODE: xxxxxx Expiration date: 2012-03-02 To redeem this offer, please purchase ab18200, test it with IHC-P with your mouse samples then submit an Abreview. Please remember to include the discount code above in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests either way. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. I look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount

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Question
Answer

DISCOUNT CODE: ******* Expiration date: ******* I am very pleased to hear you would like to accept our offer and test ab19347 in mouse tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse tissue and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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1-10 of 15 Abreviews or Q&A

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