Key features and details
- Rabbit polyclonal to calcyphosine
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-calcyphosine antibody
See all calcyphosine primary antibodies
DescriptionRabbit polyclonal to calcyphosine
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Dog
Recombinant full length protein corresponding to Human calcyphosine aa 1-189. (Expressed in E.coli)
MDAVDATMEKLRAQCLSRGASGIQGLARFFRQLDRDGSRSLDADEFRQGL AKLGLVLDQAEAEGVCRKWDRNGSGTLDLEEFLRALRPPMSQAREAVIAA AFAKLDRSGDGVVTVDDLRGVYSGRAHPKVRSGEWTEDEVLRRFLDNFDS SEKDGQVTLAEFQDYYSGVSASMNTDEEFVAMMTSAWQL
Database link: Q13938
- WB: Recombinant human calcyphosine protein; Pig spleen Tissue; HEK-293T cell lysate. IHC-P: Human prostate, kidney and breast cancer tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 21 kDa.|
FunctionCalcium-binding protein. May play a role in cellular signaling events .
Sequence similaritiesContains 4 EF-hand domains.
- Information by UniProt
- Calcyphosin antibody
- Calcyphosine 1 antibody
- Calcyphosine antibody
All lanes : Anti-calcyphosine antibody (ab231355) at 3 µg/ml
Lane 1 : Pig spleen tissue lysate.
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 21 kDa
Anti-calcyphosine antibody (ab231355) at 3 µg/ml + Recombinant human Calcyphosine protein
HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 21 kDa
Formalin-fixed, paraffin-embedded human kidney tissue stained for Calcyphosine using ab231355 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Calcyphosine using ab231355 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human prostate tissue stained for Calcyphosine using ab231355 at 20 µg/mL in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231355 has not yet been referenced specifically in any publications.