Key features and details
- Rabbit polyclonal to CalDAG-GEFI
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CalDAG-GEFI antibody
See all CalDAG-GEFI primary antibodies
DescriptionRabbit polyclonal to CalDAG-GEFI
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- IHC: Human skeletal muscle tissue. WB: Human tonsil tissue lysate.
Protein previously labeled as RASGRP2.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 69 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionFunctions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.
Tissue specificityDetected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney.
Sequence similaritiesBelongs to the RASGRP family.
Contains 2 EF-hand domains.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-GEF domain.
Developmental stageExpressed in fetal brain, lung, liver and kidney.
DomainThe N-terminal Ras-GEF domain mediates association with F-actin.
modificationsIsoform 2 is palmitoylated and myristoylated while isoform 1.
Cellular localizationCytoplasm > cytosol. Cell membrane. Cell junction > synapse > synaptosome. Cell projection > ruffle membrane. Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons.
- Information by UniProt
- Calcium and DAG-regulated antibody
- Calcium and DAG-regulated guanine nucleotide exchange factor I antibody
- Calcium-and diacylglycerol-regulated guanine nucleotide exchange factor I antibody
Paraffin-embedded human skeletal muscle tissue stained for CalDAG-GEFI using ab224377 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-CalDAG-GEFI antibody (ab224377) at 1/250 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG sp (human brain glioma cell line) cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 69 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224377 has not yet been referenced specifically in any publications.