Recombinant HRP Anti-Caldesmon/CDM antibody [E89] (ab208234)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [E89] to Caldesmon/CDM
- Suitable for: WB
- Reacts with: Mouse, Human
- Conjugation: HRP
Related conjugates and formulations
Overview
-
Product name
HRP Anti-Caldesmon/CDM antibody [E89]
See all Caldesmon/CDM primary antibodies -
Description
HRP Rabbit monoclonal [E89] to Caldesmon/CDM -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HeLa and NIH3T3 cell lysates
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E89 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- Alexa Fluor® 488 Anti-Caldesmon/CDM antibody [E89] (ab208116)
- Alexa Fluor® 647 Anti-Caldesmon/CDM antibody [E89] (ab208117)
- PE Anti-Caldesmon/CDM antibody [E89] (ab211580)
- Alexa Fluor® 555 Anti-Caldesmon/CDM antibody [E89] (ab214629)
- Anti-Caldesmon/CDM antibody [E89] - BSA and Azide free (ab215275)
- Anti-Caldesmon/CDM antibody [E89] (ab32330)
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab208234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/15000. Detects a band of approximately 75 kDa (predicted molecular weight: 93 kDa).
|
Notes |
---|
WB
1/15000. Detects a band of approximately 75 kDa (predicted molecular weight: 93 kDa). |
Target
-
Function
Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. -
Tissue specificity
High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart. -
Sequence similarities
Belongs to the caldesmon family. -
Domain
The N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form. -
Post-translational
modificationsIn non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle). - Information by UniProt
-
Database links
- Entrez Gene: 800 Human
- Entrez Gene: 109624 Mouse
- Entrez Gene: 25687 Rat
- Omim: 114213 Human
- SwissProt: Q05682 Human
- SwissProt: Q62736 Rat
- Unigene: 490203 Human
-
Alternative names
- CAD antibody
- CALD 1 antibody
- CALD1 antibody
see all
Images
-
All lanes : HRP Anti-Caldesmon/CDM antibody [E89] (ab208234) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab208234 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab208234 has not yet been referenced specifically in any publications.