Anti-Caldesmon/CDM antibody (ab183146)
Key features and details
- Rabbit polyclonal to Caldesmon/CDM
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Caldesmon/CDM antibody
See all Caldesmon/CDM primary antibodies -
Description
Rabbit polyclonal to Caldesmon/CDM -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Guinea pig, Dog, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Chinese hamster, Common marmoset, Orangutan -
Immunogen
Synthetic peptide within Human Caldesmon/CDM aa 500-600. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q05682 -
Positive control
- HeLa and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab183146 was affinity purified using an epitope specific to Caldesmon/CDM immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 93 kDa.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 93 kDa. |
Target
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Function
Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. -
Tissue specificity
High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart. -
Sequence similarities
Belongs to the caldesmon family. -
Domain
The N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form. -
Post-translational
modificationsIn non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle). - Information by UniProt
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Database links
- Entrez Gene: 472523 Chimpanzee
- Entrez Gene: 100386075 Common marmoset
- Entrez Gene: 101146534 Gorilla
- Entrez Gene: 100724959 Guinea pig
- Entrez Gene: 800 Human
- Entrez Gene: 109624 Mouse
- Entrez Gene: 100173490 Orangutan
- Entrez Gene: 25687 Rat
see all -
Alternative names
- CAD antibody
- CALD 1 antibody
- CALD1 antibody
see all
Images
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Immunoprecipitation of HeLa whole cell lysate using ab183146 at 6 µg/mg of lysate (1 mg/IP; 20% of IP loaded per lane) followed by detection of Caldesmon/CDM with ab183146 at 1 µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody.
Exposure: 10second
developed using the ECL technique
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All lanes : Anti-Caldesmon/CDM antibody (ab183146) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 93 kDa
Exposure time: 3 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab183146 has been referenced in 2 publications.
- Serres MP et al. F-Actin Interactome Reveals Vimentin as a Key Regulator of Actin Organization and Cell Mechanics in Mitosis. Dev Cell 52:210-222.e7 (2020). PubMed: 31928973
- Dadhich P et al. BioSphincters to treat Fecal Incontinence in Nonhuman Primates. Sci Rep 9:18096 (2019). PubMed: 31792260