Key features and details
- Rabbit polyclonal to Caldesmon/CDM
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Caldesmon/CDM antibody
See all Caldesmon/CDM primary antibodies
DescriptionRabbit polyclonal to Caldesmon/CDM
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Guinea pig, Dog, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Chinese hamster, Common marmoset, Orangutan
- HeLa and 293T whole cell lysates.
This product was previously labelled as Caldesmon
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab183146 was affinity purified using an epitope specific to Caldesmon/CDM immobilized on solid support.
Our Abpromise guarantee covers the use of ab183146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 93 kDa.|
FunctionActin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.
Tissue specificityHigh-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
Sequence similaritiesBelongs to the caldesmon family.
DomainThe N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.
modificationsIn non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).
- Information by UniProt
- CAD antibody
- CALD 1 antibody
- CALD1 antibody
Immunoprecipitation of HeLa whole cell lysate using ab183146 at 6 µg/mg of lysate (1 mg/IP; 20% of IP loaded per lane) followed by detection of Caldesmon/CDM with ab183146 at 1 µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody.
developed using the ECL technique
All lanes : Anti-Caldesmon/CDM antibody (ab183146) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 93 kDa
Exposure time: 3 minutes
ab183146 has been referenced in 2 publications.