Anti-Caldesmon/CDM antibody [CALD1/820] - BSA and Azide free (ab212966)

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Overview

  • Product name

    Anti-Caldesmon/CDM antibody [CALD1/820] - BSA and Azide free
    See all Caldesmon/CDM primary antibodies

  • Description

    Mouse monoclonal [CALD1/820] to Caldesmon/CDM - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human Caldesmon/CDM aa 1-793.
    Sequence:

    MDDFERRREL RRQKREEMRL EAERIAYQRN DDDEEEAARE RRRRARQERL RQKQEEESLG QVTDQVEVNA QNSVPDEEAK TTTTNTQVEG DDEAAFLERL ARREERRQKR LQEALERQKE FDPTITDASL SLPSRRMQND TAENETTEKE EKSESRQERY EIEETETVTK SYQKNDWRDA EENKKEDKEK EEEEEEKPKR GSIGENQVEV MVEEKTTESQ EETVVMSLKN GQISSEEPKQ EEEREQGSDE ISHHEKMEEE DKERAEAERA RLEAEERERI KAEQDKKIAD ERARIEAEEK AAAQERERRE AEERERMREE EKRAAEERQR IKEEEKRAAE ERQRIKEEEK RAAEERQRIK EEEKRAAEER QRARAEEEEK AKVEEQKRNK QLEEKKHAMQ ETKIKGEKVE QKIEGKWVNE KKAQEDKLQT AVLKKQGEEK GTKVQAKREK LQEDKPTFKK EEIKDEKIKK DKEPKEEVKS FMDRKKGFTE VKSQNGEFMT HKLKHTENTF SRPGGRASVD TKEAEGAPQV EAGKRLEELR RRRGETESEE FEKLKQKQQE AALELEELKK KREERRKVLE EEEQRRKQEE ADRKLREEEE KRRLKEEIER RRAEAAEKRQ KMPEDGLSDD KKPFKCFTPK GSSLKIEERA EFLNKSVQKS SGVKSTHQAA IVSKIDSRLE QYTSAIEGTK SAKPTKPAAS DLPVPAEGVR NIKSMWEKGN VFSSPTAAGT PNKETAGLKV GVSSRINEWL TKTPDGNKSP APKPSDLRPG DVSSKRNLWE KQSVDKVTSP TKV


    Database link: Q05682

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human uterus tissue

  • General notes

    ab212966 is a carrier free version of ab216011. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes.  This product was previously labelled as Caldesmon

     

Properties

Applications

Our Abpromise guarantee covers the use of ab212966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary incubation wasfor 30 minutes at RT.

 

Target

  • Function

    Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.

  • Tissue specificity

    High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.

  • Sequence similarities

    Belongs to the caldesmon family.

  • Domain

    The N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.

  • Post-translational
    modifications

    In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization.

  • Cellular localization

    Cytoplasm > cytoskeleton. Cytoplasm > myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).
  • Information by UniProt

  • Database links

    • Alternative names

      • CAD antibody
      • CALD 1 antibody
      • CALD1 antibody
      • CALD1_HUMAN antibody
      • Caldesmon 1 antibody
      • Caldesmon 1 Isoform 1 antibody
      • Caldesmon 1 Isoform 2 antibody
      • Caldesmon 1 Isoform 3 antibody
      • Caldesmon 1 Isoform 4 antibody
      • Caldesmon 1 Isoform 5 antibody
      • Caldesmon antibody
      • Caldesmon1 antibody
      • CDM antibody
      • H CAD antibody
      • HCAD antibody
      • L CAD antibody
      • LCAD antibody
      • MGC21352 antibody
      • NAG22 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon antibody (ab212966)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon antibody (ab212966)

      Immunohistochemical analysis of formalin fixed, paraffin embedded Human uterus tissue labeling Caldesmon/CDM with ab216011 at 0.5 μg/ml.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216011)

      .

    References

    ab212966 has not yet been referenced specifically in any publications.

    Publishing research using ab212966? Please let us know so that we can cite the reference in this datasheet.

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