Product nameAnti-Caldesmon/CDM (phospho S759) antibody [EPR2212]
See all Caldesmon/CDM primary antibodies
DescriptionRabbit monoclonal [EPR2212] to Caldesmon/CDM (phospho S759)
The antibody only detects Caldesmon/CDM phosphorylated at Serine 759.
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Human
corresponding to Human Caldesmon/CDM.
- HeLa lysate untreated and treated with Calyculin A
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Caldesmon
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab76106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1500 - 1/3000. Predicted molecular weight: 93 kDa.|
FunctionActin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.
Tissue specificityHigh-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
Sequence similaritiesBelongs to the caldesmon family.
DomainThe N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.
modificationsIn non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).
- Information by UniProt
- CAD antibody
- CALD 1 antibody
- CALD1 antibody
All lanes : Anti-Caldesmon/CDM (phospho S759) antibody [EPR2212] (ab76106) at 1/3000 dilution
Lane 1 : HeLa lysate, untreated
Lane 2 : HeLa lysate treated with Calyculin A
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 93 kDa
Observed band size: 93 kDa why is the actual band size different from the predicted?
ab76106 has not yet been referenced specifically in any publications.