Key features and details
- Rabbit polyclonal to CALHM2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CALHM2 antibody
DescriptionRabbit polyclonal to CALHM2
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human colon tissue; RT-4 and U-251 MG cell lysates; Human Plasma, Liver and Tonsil lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121446 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
Sequence similaritiesBelongs to the FAM26 family.
- Information by UniProt
- CAHM2_HUMAN antibody
- Calcium homeostasis modulator 2 antibody
- Calcium homeostasis modulator protein 2 antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in mitochondria. Recommended concentration of ab121446 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121446, at 1/400 dilution, staining CALHM2 in paraffin-embedded Human colon tissue by Immunohistochemistry.
All lanes : Anti-CALHM2 antibody (ab121446) at 1/250 dilution
Lane 1 : RT-4 cell lysate.
Lane 2 : U-251 MG cell lysate.
Lane 3 : Human Plasma
Lane 4 : Human liver lysate.
Lane 5 : Human tonsil lysate.
Developed using the ECL technique.
ab121446 has not yet been referenced specifically in any publications.