Overview

  • Product name

    Anti-Calmegin antibody - C-terminal
    See all Calmegin primary antibodies
  • Description

    Rabbit polyclonal to Calmegin - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse Calmegin aa 590-611 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    DESPGSGDAPLKSLRKRRVRKD


    Database link: P52194

  • Positive control

    • Mouse testis extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab191556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100.
WB 1/1000. Predicted molecular weight: 69 kDa.

Target

  • Function

    Probably plays an important role in spermatogenesis. Binds calcium ions.
  • Tissue specificity

    Testis.
  • Sequence similarities

    Belongs to the calreticulin family.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Calmegin antibody
    • Calmegin precursor antibody
    • Calnexin-T antibody
    • Clgn antibody
    • CLGN_HUMAN antibody
    • MEG 1 antigen antibody
    • OTTHUMP00000164610 antibody
    see all

Images

  • All lanes : Anti-Calmegin antibody - C-terminal (ab191556) at 1/1000 dilution

    Lane 1 : Mouse testis tissue lysate
    Lane 2 : Mouse sperm cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 69 kDa

  • Immunoprecipitation of Calmegin from mouse testis lysate (1 mg) using 2 μl of ab191556 and 50 μl of protein A conjugated magnetic beads. The immunoprecipitated sample was analyzed by western blotting with ab191556 at 1/1000 dilution.
    1. Input 2. non immune serum 3. ab191556

  • All lanes : Anti-Calmegin antibody - C-terminal (ab191556) at 1/1000 dilution

    Lane 1 : Triton X 100 extracts from wild type mouse testis
    Lane 2 : Triton X 100 extracts from Calmegin knockout mouse testis

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 69 kDa

References

This product has been referenced in:

  • Ikawa M  et al. Calsperin is a testis-specific chaperone required for sperm fertility. J Biol Chem 286:5639-46 (2011). WB, IP . Read more (PubMed: 21131354) »
  • Ikawa M  et al. Calmegin is required for fertilin alpha/beta heterodimerization and sperm fertility. Dev Biol 240:254-61 (2001). WB, IP . Read more (PubMed: 11784061) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191556.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up