Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11832-11] to Calmegin
- Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-Calmegin antibody [EPR11832-11]
See all Calmegin primary antibodies
DescriptionRabbit monoclonal [EPR11832-11] to Calmegin
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chimpanzee, Common marmoset
Recombinant fragment within Human Calmegin. The exact sequence is proprietary.
Database link: O14967
- Human testis, Mouse testis and Rat testis tissue lysate and Jurkat cell lysate; Human testis tissue; Jurkat cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab172477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 93 kDa (predicted molecular weight: 70 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionProbably plays an important role in spermatogenesis. Binds calcium ions.
Sequence similaritiesBelongs to the calreticulin family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- Calmegin antibody
- Calmegin precursor antibody
- Calnexin-T antibody
IHC-P image of Calmegin staining with ab172477 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab172477 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.
All lanes : Anti-Calmegin antibody [EPR11832-11] (ab172477) at 1/10000 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Mouse testis tissue lysate
Lane 4 : Rat testis tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 70 kDa
Immunohistochemical analysis of paraffin embedded Human testis tissue labeling Calmegin with ab172477 at 1/250 dilution.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescent analysis of Jurkat cells labeling Calmegin with ab172477 at 1/100 dilution.
Flow cytometric analysis of permeabilized Jurkat cells labeling Calmegin with ab172477 at 1/10 dilution (red) compared with a rabbit IgG (negative) (green).
Western blot analysis on immunoprecipitation pellet from Human testis lysate labeling Calmegin with ab172477 at 1/10 dilution.
ab172477 has not yet been referenced specifically in any publications.