Recombinant Anti-Calmegin antibody [EPR11832-11] (ab172477)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] (ab172477)](https://www.abcam.com/ps/products/172/ab172477/Images/ab172477-203204-anti-calmegin-antibody-epr11832-11-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] (ab172477)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11832-11] to Calmegin
- Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Calmegin antibody [EPR11832-11]
See all Calmegin primary antibodies -
Description
Rabbit monoclonal [EPR11832-11] to Calmegin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chimpanzee, Common marmoset
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Immunogen
Recombinant fragment within Human Calmegin. The exact sequence is proprietary.
Database link: O14967 -
Positive control
- Human testis, Mouse testis and Rat testis tissue lysate and Jurkat cell lysate; Human testis tissue; Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11832-11 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 93 kDa (predicted molecular weight: 70 kDa).
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| IHC-P | (2) |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/250.
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| IP |
1/10 - 1/100.
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| Flow Cyt |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
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WB
1/10000 - 1/50000. Detects a band of approximately 93 kDa (predicted molecular weight: 70 kDa). |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
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IP
1/10 - 1/100. |
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Flow Cyt
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Probably plays an important role in spermatogenesis. Binds calcium ions. -
Tissue specificity
Testis. -
Sequence similarities
Belongs to the calreticulin family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 1047 Human
- Entrez Gene: 12745 Mouse
- Entrez Gene: 685504 Rat
- Omim: 601858 Human
- SwissProt: O14967 Human
- SwissProt: P52194 Mouse
- Unigene: 86368 Human
- Unigene: 358581 Mouse
see all -
Alternative names
- Calmegin antibody
- Calmegin precursor antibody
- Calnexin-T antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] (ab172477)This image is courtesy of an abreview from Zachary Yu-Ching Lin
IHC-P image of Calmegin staining with ab172477 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab172477 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.
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![Western blot - Anti-Calmegin antibody [EPR11832-11] (ab172477)](https://www.abcam.com/ps/products/172/ab172477/Images/ab172477-194748-anti-calmegin-antibody-epr11832-11-western-blot.jpg)
Western blot - Anti-Calmegin antibody [EPR11832-11] (ab172477)All lanes : Anti-Calmegin antibody [EPR11832-11] (ab172477) at 1/10000 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Mouse testis tissue lysate
Lane 4 : Rat testis tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 70 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] (ab172477)](https://www.abcam.com/ps/products/172/ab172477/Images/ab172477-194749-anti-calmegin-antibody-epr11832-11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] (ab172477)Immunohistochemical analysis of paraffin embedded Human testis tissue labeling Calmegin with ab172477 at 1/250 dilution.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Calmegin antibody [EPR11832-11] (ab172477)](https://www.abcam.com/ps/products/172/ab172477/Images/ab172477-194751-anti-calmegin-antibody-epr11832-11-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Calmegin antibody [EPR11832-11] (ab172477)Immunofluorescent analysis of Jurkat cells labeling Calmegin with ab172477 at 1/100 dilution.
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![Flow Cytometry - Anti-Calmegin antibody [EPR11832-11] (ab172477)](https://www.abcam.com/ps/products/172/ab172477/Images/ab172477-194752-anti-calmegin-antibody-epr11832-11-flow-cytometry.jpg)
Flow Cytometry - Anti-Calmegin antibody [EPR11832-11] (ab172477)Flow cytometric analysis of permeabilized Jurkat cells labeling Calmegin with ab172477 at 1/10 dilution (red) compared with a rabbit IgG (negative) (green).
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![Immunoprecipitation - Anti-Calmegin antibody [EPR11832-11] (ab172477)](https://www.abcam.com/ps/products/172/ab172477/Images/ab172477-194754-anti-calmegin-antibody-epr11832-11-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Calmegin antibody [EPR11832-11] (ab172477)Western blot analysis on immunoprecipitation pellet from Human testis lysate labeling Calmegin with ab172477 at 1/10 dilution.
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![Anti-Calmegin antibody [EPR11832-11] (ab172477)](https://www.abcam.com/ps/products/172/ab172477/Images/ab172477-7-benefits-of-recombinant-antibodies.png)
Anti-Calmegin antibody [EPR11832-11] (ab172477)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab172477 has not yet been referenced specifically in any publications.