Overview

  • Product name
    Anti-Calmegin antibody [EPR11832]
    See all Calmegin primary antibodies
  • Description
    Rabbit monoclonal [EPR11832] to Calmegin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Common marmoset
  • Immunogen

    Recombinant fragment within Human Calmegin. The exact sequence is proprietary.
    Database link: O14967

  • Positive control
    • Human testis tissue. Jurkat cells. Jurkat, Human testis, mouse testis and rat testis lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab171971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 70 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.

Target

Images

  • IHC-P image of Calmegin staining with ab171971 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab171971 (1/500 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.

    See Abreview

  • All lanes : Anti-Calmegin antibody [EPR11832] (ab171971) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Human testis lysate
    Lane 3 : Mouse testis lysate
    Lane 4 : Rat testis lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 70 kDa

  • Immunofluorescence analysis of Jurkat cells, labeling Calmegin using ab171971 at a 1/50 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue, labeling Calmegin using ab171971 at a 1/100 dilution.

  • ab171971 showing -ve staining in Human normal brain tissue.
  • ab171971 showing -ve staining in Human skeletal muscle tissue.
  • ab171971 showing -ve staining in Human normal tonsil tissue.
  • ab171971 showing -ve staining in Human normal lung tissue.
  • Western blot analysis on immunoprecipitation pellet from Human testis lysate, labeling Calmegin using ab171971 at a 1/10 dilution and an IgG control.

References

This product has been referenced in:
  • Shang X  et al. Serine protease PRSS55 is crucial for male mouse fertility via affecting sperm migration and sperm-egg binding. Cell Mol Life Sci N/A:N/A (2018). Read more (PubMed: 30032357) »
  • Yu-Ching Lin Z  et al. Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate characteristics during testicular development. Dev Biol N/A:N/A (2015). IHC-P ; Common marmoset . Read more (PubMed: 25624265) »
See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample
Marmoset (common) Tissue sections (Adult testis)
Specification
Adult testis
Permeabilization
No
Fixative
Formaldehyde

Dr. 煜欽 林

Verified customer

Submitted Aug 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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