Western blot - Anti-Calmegin antibody [EPR11832] (ab171971)
All lanes : Anti-Calmegin antibody [EPR11832] (ab171971) at 1/10000 dilution
Lane 1 : Jurkat cell lysate Lane 2 : Human testis lysate Lane 3 : Mouse testis lysate Lane 4 : Rat testis lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 70 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832] (ab171971)This image is courtesy of an abreview from Zachary Yu-Ching Lin
IHC-P image of Calmegin staining with ab171971 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab171971 (1/500 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.
Yu-Ching Lin Z et al. Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate characteristics during testicular development. Dev BiolN/A:N/A (2015).
Read more (PubMed: 25624265) »