Anti-CALML3 antibody (ab230240)
Key features and details
- Rabbit polyclonal to CALML3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CALML3 antibody
See all CALML3 primary antibodies -
Description
Rabbit polyclonal to CALML3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Recombinant full length protein corresponding to Human CALML3 aa 1 to the C-terminus.
Database link: P27482 -
Positive control
- WB: A431 whole cell lysate. IHC-P: Human cervical cancer and skin tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230240 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 17 kDa. |
IHC-P
1/20 - 1/200. |
Target
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Function
May be similar to that of authentic calmodulin and may actually compete with calmodulin by binding, with different affinities, to cellular substrates. -
Tissue specificity
Expressed in normal mammary, prostate, cervical, and epidermal tissues. It is greatly reduced or undetectable in transformed cells. -
Sequence similarities
Belongs to the calmodulin family.
Contains 4 EF-hand domains. - Information by UniProt
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Database links
- Entrez Gene: 810 Human
- Entrez Gene: 307100 Rat
- Omim: 114184 Human
- SwissProt: P27482 Human
- SwissProt: Q5U206 Rat
- Unigene: 239600 Human
- Unigene: 105124 Rat
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Alternative names
- CALL3_HUMAN antibody
- CALML3 antibody
- Calmodulin like 3 antibody
see all
Images
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Anti-CALML3 antibody (ab230240) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa -
Paraffin-embedded human skin tissue stained for CALML3 using ab230240 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human cervical cancer tissue stained for CALML3 using ab230240 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab230240 has not yet been referenced specifically in any publications.