Overview

  • Product name

  • Description

    Rabbit polyclonal to CALML5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: GELTPEEEAQ YKKAFSAVDT DGNGTINAQE LGAALKATGK NLSEAQLRKL ISEVDGDGDG EISFQEFLTA, corresponding to N terminal amino acids 3-72 of Human CALML5.

  • Positive control

    • Human skin tissue. CALML5 over-expression lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab122665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function

    Binds calcium. May be involved in terminal differentiation of keratinocytes.
  • Tissue specificity

    Particularly abundant in the epidermis where its expression is directly related to keratinocyte differentiation. Very low expression in lung.
  • Sequence similarities

    Contains 4 EF-hand domains.
  • Information by UniProt
  • Database links

  • Alternative names

    • CALL5_HUMAN antibody
    • CALML5 antibody
    • Calmodulin like 5 antibody
    • Calmodulin-like protein 5 antibody
    • Calmodulin-like skin protein antibody
    • CLSP antibody
    see all

Images

  • ab122665 at 1/2500 dilution staining CALML5 in paraffin-embedded Human skin tissue by Immunohistochemistry.
  • Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab122665 1-4 µg/ml. Cells treated with PFA/Triton X-100.

References

ab122665 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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