- Datasheet
- Specific References (6)
- Protocols
Overview
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Product nameAnti-Calmodulin 1/2/3 antibody [2D1]
See all Calmodulin 1/2/3 primary antibodies -
DescriptionMouse monoclonal [2D1] to Calmodulin 1/2/3
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Host speciesMouse
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SpecificityThis antibody detects calmodulin. It does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK).By Western blot, this antibody detects a 17 kDa protein representing calmodulin from Dictyostelium cell lysate. Immunohistochemical staining of calmodulin in Dictyostelium cells with this antibody results in staining of the contractile vacuoles.
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Tested applicationsSuitable for: Flow Cyt, ELISA, WB, IHC-P, ICC, IP, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Chicken, Cow, Human, Dictyostelium discoideum
Predicted to work with: Wheat
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Immunogen
Other Immunogen Type corresponding to Calmodulin 1/2/3. Calmodulin purified from Dictyostelium discoideum.
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Positive control
- WB: Purified Dictyostelium calmodulin IHC: rat brain
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General notes
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityProtein A purified
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Primary antibody notesCalmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations.
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ClonalityMonoclonal
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Clone number2D1
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab2860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| Flow Cyt | Use 2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| ELISA | Use at an assay dependent concentration. | |
| WB | 1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). | |
| IHC-P | 1/20. | |
| ICC | 1/50. | |
| IP | Use at an assay dependent concentration. | |
| ICC/IF | 1/20. |
Target
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RelevanceFunction: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
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Cellular localizationCytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
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Database links
- Entrez Gene: 416692 Chicken
- Entrez Gene: 326597 Cow
- Entrez Gene: 520277 Cow
- Entrez Gene: 808 Human
- Entrez Gene: 12315 Mouse
- Entrez Gene: 24244 Rat
- Omim: 114182 Human
- Omim: 114183 Human
see all -
FormThere are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase.
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Alternative names
- CALM 1 antibody
- CALM 2 antibody
- CALM 3 antibody
see all
Images
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![Flow Cytometry - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)](https://www.abcam.com/ps/products/2/ab2860/Images/ab2860-11-ab2860FACS3.jpg)
Flow Cytometry - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)Flow cytometry analysis of Calmodulin showing positive staining in the cytoplasm of PC12 cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant and adding 90% methanol followed by incubation for 10 minutes at room temperature. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2860 at 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis. -
![Flow Cytometry - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)](https://www.abcam.com/ps/products/2/ab2860/Images/ab2860-10-ab2860FACS2.jpg)
Flow Cytometry - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)Flow cytometry analysis of Calmodulin showing positive staining in the cytoplasm of MCF-7 cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant and adding 90% methanol followed by incubation for 10 minutes at room temperature. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2860 at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis. -
![Flow Cytometry - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)](https://www.abcam.com/ps/products/2/ab2860/Images/ab2860-9-ab2860FACS1.jpg)
Flow Cytometry - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)Flow cytometry analysis of Calmodulin showing positive staining in the cytoplasm of C6 cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant and adding 90% methanol followed by incubation for 10 minutes at room temperature. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2860 at 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)](https://www.abcam.com/ps/products/2/ab2860/Images/ab2860_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)Immunolocalization of calmodulin in rat brain using ab2860 (1:100) -
![Western blot - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)](https://www.abcam.com/ps/products/2/ab2860/Images/ab2860_1.jpg)
Western blot - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860) at 1/500 dilution + Purified Dictyostelium calmodulin
Predicted band size: 17 kDa -
![Flow Cytometry - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)](https://www.abcam.com/ps/products/2/ab2860/Images/Calmodulin-Primary-antibodies-ab2860-6.jpg)
Flow Cytometry - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)Overlay histogram showing HeLa cells stained with ab2860 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2860, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. -
![Immunocytochemistry/ Immunofluorescence - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)](https://www.abcam.com/ps/products/2/ab2860/Images/Calmodulin-Primary-antibodies-ab2860-7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)Immunofluorescent analysis of Calmodulin using Calmodulin Monoclonal antibody (2D1) ab2860 shows staining in HeLa cells. Calmodulin staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin ab2860 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification. -
![Immunocytochemistry/ Immunofluorescence - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)](https://www.abcam.com/ps/products/2/ab2860/Images/Calmodulin-Primary-antibodies-ab2860-8.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)Immunofluorescent analysis of Calmodulin using Calmodulin Monoclonal antibody (2D1) ab2860 shows staining in A2058 melanoma cells. Calmodulin staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin ab2860 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification. -
![Immunocytochemistry/ Immunofluorescence - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)](https://www.abcam.com/ps/products/2/ab2860/Images/Calmodulin-Primary-antibodies-ab2860-9.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)Immunofluorescent analysis of Calmodulin using Calmodulin Monoclonal antibody (2D1) ab2860 shows staining in C6 glioma cells. Calmodulin staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin ab2860 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)](https://www.abcam.com/ps/products/2/ab2860/Images/Calmodulin-Primary-antibodies-ab2860-10.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)Immunohistochemistry was performed on normal biopsies of deparaffinized Rat testis tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Calmodulin ab2860 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)](https://www.abcam.com/ps/products/2/ab2860/Images/Calmodulin-Primary-antibodies-ab2860-11.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 antibody [2D1] (ab2860)Immunohistochemistry was performed on normal biopsies of deparaffinized Rat cerebellum tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Calmodulin ab2860 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Protocols
References
This product has been referenced in:
- Li T et al. The interactome and spatial redistribution feature of Ca2+receptor protein calmodulin reveals a novel role in invadopodia-mediated invasion. Cell Death Dis 9:292 (2018). Read more (PubMed: 29463791) »
- Salman MM et al. Hypothermia increases aquaporin 4 (AQP4) plasma membrane abundance in human primary cortical astrocytes via a calcium/transient receptor potential vanilloid 4 (TRPV4)- and calmodulin-mediated mechanism. Eur J Neurosci 46:2542-2547 (2017). ELISA . Read more (PubMed: 28925524) »
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