Other product details
Host species: Goat
Conjugation: Alkaline Phosphatase
There is a very faint band in the hCam and the Cam2 samples, however these developed after 4 hours and are much less intense than seen on the coomassie gel.
Varying conditions and concentrations were used, (1/5000, 1/2500, 1/1000, 1/500 and 1/250) and blocking with milk/BSA and also using TBST or PBST. The above gave the only results.
Even the human CaM band is not very clear. This cannot be used as a way to detect any calmodulin in fission yeast.
The reviewer has confirmed that a Coomassie stain of the S. pombe recombinant Cam2 preparation in an SDS-PAGE gel shows a primary band at 17 kDa that is more intense than other bands, indicating a higher purity than the blot suggests.
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Submitted Mar 09 2011