Overview

  • Product name
    Anti-Calmodulin 1/2/3 antibody
    See all Calmodulin 1/2/3 primary antibodies
  • Description
    Rabbit polyclonal to Calmodulin 1/2/3
  • Host species
    Rabbit
  • Specificity
    Targets C terminusof Calmodulin.
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Calmodulin 1/2/3 aa 10-30 (C terminal) conjugated to keyhole limpet haemocyanin.

  • Positive control
    • Ramos cell lysate; Human breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab38590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    Function: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
  • Cellular localization
    Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
  • Database links
  • Form
    There are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase.
  • Alternative names
    • CALM 1 antibody
    • CALM 2 antibody
    • CALM 3 antibody
    • CALM antibody
    • CALM1 antibody
    • CALM2 antibody
    • CALM3 antibody
    • CALML2 antibody
    • calmodulin 1 (phosphorylase kinase, delta) antibody
    • Calmodulin 1 antibody
    • Calmodulin 2 (phosphorylase kinase, delta) antibody
    • Calmodulin 2 antibody
    • Calmodulin 3 (phosphorylase kinase, delta) antibody
    • Calmodulin 3 antibody
    • CAM 2 antibody
    • CAM 3 antibody
    • CAM I antibody
    • CAM1 antibody
    • CAM2 antibody
    • CAM3 antibody
    • CAMB antibody
    • CAMC antibody
    • CAMI antibody
    • CAMII antibody
    • CAMIII antibody
    • CPVT4 antibody
    • DD132 antibody
    • FLJ99410 antibody
    • LP7057 protein antibody
    • PHKD antibody
    • PHKD2 antibody
    • PHKD3 antibody
    • phosphorylase kinase delta antibody
    • phosphorylase kinase, delta subunit antibody
    see all

Images

  • Anti-Calmodulin 1/2/3 antibody (ab38590) + Ramos cell lysate

    Predicted band size: 17 kDa
    Observed band size: 17 kDa
    Additional bands at: 25 kDa, 30 kDa. We are unsure as to the identity of these extra bands.

  • Formalin-fixed paraffin-embedded human cancer tissue (breast carcinoma) with ab38590 anti-calmodulin, peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References

This product has been referenced in:
  • Salman MM  et al. Hypothermia increases aquaporin 4 (AQP4) plasma membrane abundance in human primary cortical astrocytes via a calcium/transient receptor potential vanilloid 4 (TRPV4)- and calmodulin-mediated mechanism. Eur J Neurosci 46:2542-2547 (2017). ELISA . Read more (PubMed: 28925524) »
  • Miyoshi N  et al. Nodes-and-connections RNAi knockdown screening: identification of a signaling molecule network involved in fulvestrant action and breast cancer prognosis. Oncogenesis 4:e172 (2015). IHC . Read more (PubMed: 26479444) »

See all 4 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (sarcoma)
Specification
sarcoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6, 20 min, 100C
Permeabilization
No
Username

Abcam user community

Verified customer

Submitted Dec 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up