Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742)


  • Product name
    Anti-Calmodulin 1/2/3 antibody [EPR5028]
    See all Calmodulin 1/2/3 primary antibodies
  • Description
    Rabbit monoclonal [EPR5028] to Calmodulin 1/2/3
  • Host species
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human Calmodulin 1/2/3 aa 1-100 (N terminal).

  • Positive control
    • HeLa, Ramos, HCT116 and NIH 3T3 cell lysates.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.


Our Abpromise guarantee covers the use of ab124742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Relevance
      Function: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
    • Cellular localization
      Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
    • Database links
    • Form
      There are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase.
    • Alternative names
      • CALM 1 antibody
      • CALM 2 antibody
      • CALM 3 antibody
      • CALM antibody
      • CALM1 antibody
      • CALM2 antibody
      • CALM3 antibody
      • CALML2 antibody
      • calmodulin 1 (phosphorylase kinase, delta) antibody
      • Calmodulin 1 antibody
      • Calmodulin 2 (phosphorylase kinase, delta) antibody
      • Calmodulin 2 antibody
      • Calmodulin 3 (phosphorylase kinase, delta) antibody
      • Calmodulin 3 antibody
      • CAM 2 antibody
      • CAM 3 antibody
      • CAM I antibody
      • CAM1 antibody
      • CAM2 antibody
      • CAM3 antibody
      • CAMB antibody
      • CAMC antibody
      • CAMI antibody
      • CAMII antibody
      • CAMIII antibody
      • CPVT4 antibody
      • DD132 antibody
      • FLJ99410 antibody
      • LP7057 protein antibody
      • PHKD antibody
      • PHKD2 antibody
      • PHKD3 antibody
      • phosphorylase kinase delta antibody
      • phosphorylase kinase, delta subunit antibody
      see all


    • Overlay histogram showing SHSY-5Y cells stained with ab124742 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124742, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742) at 1/50000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Ramos cell lysate
      Lane 3 : HCT116 cell lysate
      Lane 4 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 17 kDa
      Observed band size: 17 kDa


    This product has been referenced in:
    • Yang Y  et al. Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP. Nat Commun 9:1504 (2018). ICC/IF . Read more (PubMed: 29666364) »
    • Simms BA  et al. A novel calmodulin site in the Cav1.2 N-terminus regulates calcium-dependent inactivation. Pflugers Arch 466:1793-803 (2014). WB ; Rat . Read more (PubMed: 24352630) »

    See all 2 Publications for this product

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