Recombinant Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742)
![Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742)](https://www.abcam.com/ps/products/124/ab124742/Images/ab124742-215882-anti-calmodulin-1-2-3-antibody-epr5028-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5028] to Calmodulin 1/2/3
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Calmodulin 1/2/3 antibody [EPR5028]
See all Calmodulin 1/2/3 primary antibodies -
Description
Rabbit monoclonal [EPR5028] to Calmodulin 1/2/3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Ramos, HCT116 and NIH 3T3 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5028 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/50000 - 1/200000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
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| Flow Cyt (Intra) |
1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
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WB
1/50000 - 1/200000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). |
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Flow Cyt (Intra)
1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
Function: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. -
Cellular localization
Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. -
Database links
- Entrez Gene: 808 Human
- Entrez Gene: 12315 Mouse
- Entrez Gene: 24244 Rat
- Omim: 114182 Human
- Omim: 114183 Human
- SwissProt: 114180 Human
- SwissProt: P0DP23 Human
- SwissProt: P0DP24 Human
see all -
Form
There are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase. -
Alternative names
- CALM 1 antibody
- CALM 2 antibody
- CALM 3 antibody
see all
Images
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Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742)
Overlay histogram showing SHSY-5Y cells stained with ab124742 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124742, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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![Western blot - Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742)](https://www.abcam.com/ps/products/124/ab124742/Images/ab124742-138584-anti-calmodulin-1-2-3-antibody-epr5028-western-blot.jpg)
Western blot - Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742)All lanes : Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : HCT116 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa -
![Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742)](https://www.abcam.com/ps/products/124/ab124742/Images/ab124742-3-benefits-of-recombinant-antibodies.png)
Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab124742 has been referenced in 4 publications.
- Yang Y et al. Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP. Nat Commun 9:1504 (2018). ICC/IF . PubMed: 29666364
- Zhou P et al. Mechanism of nitric oxide and acid-sensing ion channel 1a modulation of panic-like behaviour in the dorsal periaqueductal grey of the mouse. Behav Brain Res 353:32-39 (2018). PubMed: 29953907
- Bond R et al. The unique C terminus of the calcineurin isoform CNAß1 confers non-canonical regulation of enzyme activity by Ca2+ and calmodulin. J Biol Chem 292:16709-16721 (2017). PubMed: 28842480
- Simms BA et al. A novel calmodulin site in the Cav1.2 N-terminus regulates calcium-dependent inactivation. Pflugers Arch 466:1793-803 (2014). WB ; Rat . PubMed: 24352630