Product nameAnti-Calmodulin 1/2/3 (phospho T79 + S81) antibody
See all Calmodulin 1/2/3 primary antibodies
DescriptionRabbit polyclonal to Calmodulin 1/2/3 (phospho T79 + S81)
SpecificityDetects endogenous levels of Calmodulin only when phosphorylated at threonine 79 and serine 81.
Tested applicationsSuitable for: ICC/IF, ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human Calmodulin 1/2/3. The antiserum was produced against synthesized phosphopeptide derived from human Calmodulin around the phosphorylation site of threonine 79 and serine 81 (K-D-T(p)-D-S(p)-E-E)
Database link: P0DP23
- IHC-P: human brain tissue. WB: extracts from Jurkat cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab61001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/500.|
|WB||1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).|
|IHC-P||1/50 - 1/100.|
RelevanceFunction: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
Cellular localizationCytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
FormThere are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase.
- CALM 1 antibody
- CALM 2 antibody
- CALM 3 antibody
All lanes : Anti-Calmodulin 1/2/3 (phospho T79 + S81) antibody (ab61001) at 1/500 dilution
Lane 1 : HeLa cells treated with Hu (1mg/ml)
Lane 2 : Raw264.7 cells treated with TNFa (1mg/ml)
Lysates/proteins at 15 µl per lane.
Predicted band size: 17 kDa
ab61001, at a 1/50 dilution, staining bi-phosphorylated Calmodulin on human brain tissue sections, in the presence (right) or absence (left) of the immunizing phosphopeptide.
Immunofluorescence analysis of HepG2 cells labeling Calmodulin (Phospho-Thr79+Ser81) with ab61001 at 1/100 dilution. The panel on the left is without the blocking peptide, while the panel on the right includes the blocking peptide.
This product has been referenced in:
- Hamilton S et al. PKA phosphorylation underlies functional recruitment of sarcolemmal SK2 channels in ventricular myocytes from hypertrophic hearts. J Physiol N/A:N/A (2019). Read more (PubMed: 30771223) »
- Pachuau J et al. Protein kinase CK2 contributes to diminished small conductance Ca2+-activated K+ channel activity of hypothalamic pre-sympathetic neurons in hypertension. J Neurochem 130:657-67 (2014). Read more (PubMed: 24806793) »