Anti-Calnexin antibody (ab227310)
Key features and details
- Rabbit polyclonal to Calnexin
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Calnexin antibody
See all Calnexin primary antibodies -
Description
Rabbit polyclonal to Calnexin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Dog, Pig -
Immunogen
Recombinant fragment within Human Calnexin (internal sequence). The exact sequence is proprietary.
Database link: P27824 -
Positive control
- WB: HEK-293T, PC-12 and Rat2 whole cell extracts; HEK-293T, A431, HeLa and HepG2 whole cell lysate (ab7900); Mouse liver lysate. IHC-P: SW480 xenograft and human breast carcinoma tissues. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/10000. Predicted molecular weight: 68 kDa.
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Notes |
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ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/10000. Predicted molecular weight: 68 kDa. |
Target
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Function
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
Sequence similarities
Belongs to the calreticulin family. -
Cellular localization
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 416288 Chicken
- Entrez Gene: 407129 Cow
- Entrez Gene: 403908 Dog
- Entrez Gene: 821 Human
- Entrez Gene: 12330 Mouse
- Entrez Gene: 100519184 Pig
- Entrez Gene: 29144 Rat
- Omim: 114217 Human
see all -
Alternative names
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
Images
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All lanes : Anti-Calnexin antibody (ab227310) at 1/10000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lanes 2-3 : Calnexin shRNA transfected HEK-293T whole cell extract
Lysates/proteins at 15 µg per lane.
Predicted band size: 68 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-Calnexin antibody (ab227310) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded SW480 (human) xenograft tissue stained for Calnexin using ab227310 at 1/500 dilution in immunohistochemical analysis.
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Anti-Calnexin antibody (ab227310) at 1/1000 dilution + Mouse liver lysate at 50 µg
Predicted band size: 68 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-Calnexin antibody (ab227310) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa7.5% SDS-PAGE gel.
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Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Calnexin (green) using ab227310 at 1/200 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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Paraffin-embedded human breast carcinoma tissue stained for Calnexin using ab227310 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab227310 has been referenced in 2 publications.
- Tong L et al. Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota. Theranostics 11:8570-8586 (2021). PubMed: 34373759
- Wang G et al. A Panel of Exosome-Derived miRNAs of Cerebrospinal Fluid for the Diagnosis of Moyamoya Disease. Front Neurosci 14:548278 (2020). PubMed: 33100957