Key features and details
- Rabbit polyclonal to Calnexin
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Calnexin antibody
See all Calnexin primary antibodies
DescriptionRabbit polyclonal to Calnexin
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide within Human Calnexin aa 1-50. The exact sequence is proprietary. NP_001737.1
Database link: P27824
- IHC-P: Human breast carcinoma tissue. WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HeLa whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab265602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 68 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionCalcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.
Sequence similaritiesBelongs to the calreticulin family.
Cellular localizationEndoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
All lanes : Anti-Calnexin antibody (ab265602) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-239T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Predicted band size: 68 kDa
Exposure time: 3 seconds
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling Calnexin with ab265602 at 1/1000 dilution. Detection: DAB.
Calnexin was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab265602 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab265602 at 1 µg/ml.
Lane 1: ab265602 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 1 sec.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab265602 has not yet been referenced specifically in any publications.