Product nameAnti-Calnexin antibody
See all Calnexin primary antibodies
DescriptionRabbit polyclonal to Calnexin
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Dog, Orangutan
Synthetic peptide within Human Calnexin aa 542-592. The exact sequence is proprietary.
Database link: P27824
- IHC-P: Human breast carcinoma tissue. IP: HeLa whole cell lysate. WB: HeLa, HEK-293T, NIH/3T3 and Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab265604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 68 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionCalcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.
Sequence similaritiesBelongs to the calreticulin family.
Cellular localizationEndoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
All lanes : Anti-Calnexin antibody (ab265604) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH/3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 68 kDa
Exposure time: 10 seconds
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling Calnexin with ab265604 at 1/1000 dilution. Detection: DAB.
Calnexin was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab265604 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab265604 at 1 µg/ml.
Lane 1: ab265604 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 3 secs.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab265604 has not yet been referenced specifically in any publications.