Overview

  • Product name

    Anti-Calnexin antibody - C-terminal
    See all Calnexin primary antibodies
  • Description

    Goat polyclonal to Calnexin - C-terminal
  • Host species

    Goat
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Dog, Human, African green monkey
  • Immunogen

    Recombinant fragment within Human Calnexin aa 550-592 (C terminal). The exact sequence is proprietary. Produced in E. coli.
    Sequence:

    E KQKSDAEEDG GTVSQEEEDR KPKAEEDEIL NRSPRNRKPR RE


    Database link: P27824

  • Positive control

    • Human (293A, primary fibroblasts, HaCat, HeLa, HMEC1, Jurkat, MNT1, U118); Rat (TR-iBRB); Mouse (3T3, AtT-20, Hepa, RAW 264.7), Monkey (COS7) and Dog (D17) whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 20% Glycerol, 79% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/500.
WB 1/500 - 1/2000. Predicted molecular weight: 68 kDa.

Target

  • Function

    Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.
  • Sequence similarities

    Belongs to the calreticulin family.
  • Cellular localization

    Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • Calnexin antibody
    • CALX_HUMAN antibody
    • CANX antibody
    • CNX antibody
    • FLJ26570 antibody
    • Histocompatibility complex class I antigen binding protein p88 antibody
    • IP90 antibody
    • Major histocompatibility complex class I antigen-binding protein p88 antibody
    • p90 antibody
    see all

Images

  • Immunofluorescent analysis of Hepa1-6 cells (4% PFA-fixed) labeling Calnexin with ab192439 at 1/100 dilution.

  • All lanes : Anti-Calnexin antibody - C-terminal (ab192439) at 1/500 dilution

    Lane 1 : 3T3 cell lysate
    Lane 2 : RAW 264.7 cell lysate
    Lane 3 : Hepa cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : rabbit anti-goat HRP at 1/10000 dilution

    Predicted band size: 68 kDa

References

This product has been referenced in:

  • Haka AS  et al. Exocytosis of macrophage lysosomes leads to digestion of apoptotic adipocytes and foam cell formation. J Lipid Res 57:980-92 (2016). IF . Read more (PubMed: 27044658) »
See 1 Publication for this product

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