Key features and details
- Goat polyclonal to Calnexin - C-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Dog, Human, African green monkey
- Isotype: IgG
Product nameAnti-Calnexin antibody - C-terminal
See all Calnexin primary antibodies
DescriptionGoat polyclonal to Calnexin - C-terminal
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Dog, Human, African green monkey
- Human (293A, primary fibroblasts, HaCat, HeLa, HMEC1, Jurkat, MNT1, U118); Rat (TR-iBRB); Mouse (3T3, AtT-20, Hepa, RAW 264.7), Monkey (COS7) and Dog (D17) whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 20% Glycerol, 79% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab192439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/500.|
|WB||1/500 - 1/2000. Predicted molecular weight: 68 kDa.|
FunctionCalcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.
Sequence similaritiesBelongs to the calreticulin family.
Cellular localizationEndoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
Immunofluorescent analysis of Hepa1-6 cells (4% PFA-fixed) labeling Calnexin with ab192439 at 1/100 dilution.
All lanes : Anti-Calnexin antibody - C-terminal (ab192439) at 1/500 dilution
Lane 1 : 3T3 cell lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : Hepa cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : rabbit anti-goat HRP at 1/10000 dilution
Predicted band size: 68 kDa
ab192439 has been referenced in 1 publication.
- Haka AS et al. Exocytosis of macrophage lysosomes leads to digestion of apoptotic adipocytes and foam cell formation. J Lipid Res 57:980-92 (2016). IF . PubMed: 27044658