Anti-Calnexin antibody - C-terminal (ab219644)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Calnexin antibody - C-terminal
See all Calnexin primary antibodies -
Description
Goat polyclonal to Calnexin - C-terminal -
Host species
Goat -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human Calnexin aa 555-592 (C terminal). The exact sequence is proprietary. Produced in E. coli.
Sequence:DAEEDGGTVSQEEEDRKPKAEEDEILNRSPRNRKPRRE
Database link: P27824 -
Positive control
- Human prostate and kidney tissues; Hepa1-6 cells; NIH 3T3, RAW 264.7 and Hepa1-6 cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 79% PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab219644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| ICC/IF | 1/50 - 1/500. | |
| WB | 1/500 - 1/2000. Predicted molecular weight: 68 kDa. |
Target
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Function
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
Sequence similarities
Belongs to the calreticulin family. -
Cellular localization
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 821 Human
- Entrez Gene: 12330 Mouse
- Omim: 114217 Human
- SwissProt: P27824 Human
- SwissProt: P35564 Mouse
- Unigene: 567968 Human
- Unigene: 248827 Mouse
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Alternative names
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
Images
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Western blot - Anti-Calnexin antibody - C-terminal (ab219644)All lanes : Anti-Calnexin antibody - C-terminal (ab219644) at 1/500 dilution
Lane 1 : NIH 3T3 cell lysates
Lane 2 : RAW 264.7 cell lysates
Lane 3 : Hepa1-6 cell lysates
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Rabbit polyclonal to goat IgG (HRP) at 1/10000 dilution
Predicted band size: 68 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - C-terminal (ab219644)Immunofluorescent analysis of Hepa1-6 cells (4% of PFA-fixed) labeling Calnexin with ab219644 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody - C-terminal (ab219644)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling Calnexin with ab219644 at 5 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody - C-terminal (ab219644)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling Calnexin with ab219644 at 5 µg/ml.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Du T et al. The deubiquitylase OTUD3 stabilizes GRP78 and promotes lung tumorigenesis. Nat Commun 10:2914 (2019). Read more (PubMed: 31266968) »
