Key features and details
- Goat polyclonal to Calnexin - C-terminal
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Calnexin antibody - C-terminal
See all Calnexin primary antibodies
DescriptionGoat polyclonal to Calnexin - C-terminal
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
- Human prostate and kidney tissues; Hepa1-6 cells; NIH 3T3, RAW 264.7 and Hepa1-6 cell lysates.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 79% PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab219644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||1/50 - 1/500.|
|WB||1/500 - 1/2000. Predicted molecular weight: 68 kDa.|
FunctionCalcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.
Sequence similaritiesBelongs to the calreticulin family.
Cellular localizationEndoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
All lanes : Anti-Calnexin antibody - C-terminal (ab219644) at 1/500 dilution
Lane 1 : NIH 3T3 cell lysates
Lane 2 : RAW 264.7 cell lysates
Lane 3 : Hepa1-6 cell lysates
Lysates/proteins at 50 µg per lane.
All lanes : Rabbit polyclonal to goat IgG (HRP) at 1/10000 dilution
Predicted band size: 68 kDa
Immunofluorescent analysis of Hepa1-6 cells (4% of PFA-fixed) labeling Calnexin with ab219644 at 1/100 dilution.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling Calnexin with ab219644 at 5 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling Calnexin with ab219644 at 5 µg/ml.
ab219644 has been referenced in 1 publication.
- Du T et al. The deubiquitylase OTUD3 stabilizes GRP78 and promotes lung tumorigenesis. Nat Commun 10:2914 (2019). PubMed: 31266968