Anti-Calnexin antibody - C-terminal (ab219644)
Key features and details
- Goat polyclonal to Calnexin - C-terminal
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Calnexin antibody - C-terminal
See all Calnexin primary antibodies -
Description
Goat polyclonal to Calnexin - C-terminal -
Host species
Goat -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human Calnexin aa 555-592 (C terminal). The exact sequence is proprietary. Produced in E. coli.
Sequence:DAEEDGGTVSQEEEDRKPKAEEDEILNRSPRNRKPRRE
Database link: P27824 -
Positive control
- Human prostate and kidney tissues; Hepa1-6 cells; NIH 3T3, RAW 264.7 and Hepa1-6 cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 79% PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab219644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| ICC/IF | 1/50 - 1/500. | |
| WB | 1/500 - 1/2000. Predicted molecular weight: 68 kDa. |
Target
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Function
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
Sequence similarities
Belongs to the calreticulin family. -
Cellular localization
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 821 Human
- Entrez Gene: 12330 Mouse
- Omim: 114217 Human
- SwissProt: P27824 Human
- SwissProt: P35564 Mouse
- Unigene: 567968 Human
- Unigene: 248827 Mouse
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Alternative names
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
Images
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Western blot - Anti-Calnexin antibody - C-terminal (ab219644)All lanes : Anti-Calnexin antibody - C-terminal (ab219644) at 1/500 dilution
Lane 1 : NIH 3T3 cell lysates
Lane 2 : RAW 264.7 cell lysates
Lane 3 : Hepa1-6 cell lysates
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Rabbit polyclonal to goat IgG (HRP) at 1/10000 dilution
Predicted band size: 68 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - C-terminal (ab219644)Immunofluorescent analysis of Hepa1-6 cells (4% of PFA-fixed) labeling Calnexin with ab219644 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody - C-terminal (ab219644)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling Calnexin with ab219644 at 5 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody - C-terminal (ab219644)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling Calnexin with ab219644 at 5 µg/ml.
Protocols
Datasheets and documents
References (1)
ab219644 has been referenced in 1 publication.
- Du T et al. The deubiquitylase OTUD3 stabilizes GRP78 and promotes lung tumorigenesis. Nat Commun 10:2914 (2019). PubMed: 31266968
