Anti-Calnexin antibody [CANX/1541] - N-terminal (ab218337)
Key features and details
- Mouse monoclonal [CANX/1541] to Calnexin - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Calnexin antibody [CANX/1541] - N-terminal
See all Calnexin primary antibodies -
Description
Mouse monoclonal [CANX/1541] to Calnexin - N-terminal -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Does not react with: Rat -
Immunogen
Recombinant fragment within Human Calnexin (N terminal). The exact sequence is proprietary.
Database link: P27824 -
Positive control
- IHC-P: Human renal cell carcinoma and Human small intestinal carcinoma tissues; WB: HeLa and MCF7 cell lysates. Human kidney tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
CANX/1541 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 68 kDa.
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Notes |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 68 kDa. |
Target
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Function
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
Sequence similarities
Belongs to the calreticulin family. -
Cellular localization
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 821 Human
- Omim: 114217 Human
- SwissProt: P27824 Human
- Unigene: 567968 Human
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Alternative names
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human renal cell carcinoma tissue labeling Calnexin with ab218337 at 2 μg/ml dilution, followed by conjugation to the secondary antibody.
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Anti-Calnexin antibody [CANX/1541] - N-terminal (ab218337) at 2 µg/ml + Human kidney tissue lysate
Predicted band size: 68 kDa -
All lanes : Anti-Calnexin antibody [CANX/1541] - N-terminal (ab218337) at 2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Predicted band size: 68 kDa -
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human small intestinal carcinoma tissue labeling Calnexin with ab218337 at 2 μg/ml dilution, followed by conjugation to the secondary antibody.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human renal cell carcinoma tissue labeling Calnexin with ab218337 at 2 μg/ml dilution, followed by conjugation to the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab218337 has not yet been referenced specifically in any publications.